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Expression of LFA-1 by a lymphoblastoid cell line from a patient with monosomy 21: effects on intercellular adhesion.
[monosomy 21]
Monosomy
21
(
M
21
)
is
a
rare
aneuploid
condition
which
in
certain
cases
leads
to
reduced
levels
of
chromosome
21
gene
products
.
We
have
prepared
an
Epstein-
Barr
virus
lymphoblastoid
cell-line
(
LCL
)
from
patient
with
M
21
who
has
immunological
abnormalities
,
and
analysed
the
expression
of
lymphocyte
function-associated
antigen-
1
(
LFA-
1
)
.
This
heterodimeric
leucocyte
integrin
consists
of
CD
11
a
(
alpha
)
subunits
non-covalently
associated
with
CD
18
(
beta
)
subunits
coded
,
respectively
,
by
genes
on
chromosomes
16
and
21
.
To
determine
whether
monosomy
21
results
in
decreased
expression
of
LFA-
1
,
monoclonal
antibodies
were
used
to
compare
the
expression
of
CD
11
a
and
CD
18
on
the
M
21
LCL
with
LCL
from
trisomy
21
(
Down
's
syndrome
,
T
21
)
,
normal
controls
and
a
possible
case
of
leucocyte
adhesion
deficiency
.
In
addition
,
phorbol-
ester
-induced
homotypic
adhesion
,
an
LFA-
1
-
mediated
effect
,
was
compared
in
these
LCLs
.
The
results
are
consistent
with
a
gene
dosage
mediated
reduction
of
LFA-
1
expression
by
the
M
21
LCL
.
