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A random Abstract
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A novel model of human skin pressure ulcers in mice.
[severe combined immunodeficiency]
Pressure
ulcers
are
a
prevalent
health
problem
in
today
's
society
.
The
shortage
of
suitable
animal
models
limits
our
understanding
and
our
ability
to
develop
new
therapies
.
This
study
aims
to
report
on
the
development
of
a
novel
and
reproducible
human
skin
pressure
ulcer
model
in
mice
.
Male
non-obese
,
diabetic
,
severe
combined
immunodeficiency
mice
(
n
 
=
 
22
)
were
engrafted
with
human
skin
.
A
full-thickness
skin
graft
was
placed
onto
4
×
3
cm
wounds
created
on
the
dorsal
skin
of
the
mice
.
Two
groups
with
permanent
grafts
were
studied
after
60
days
.
The
control
group
(
n
 
=
 
6
)
was
focused
on
the
process
of
engraftment
.
Evaluations
were
conducted
with
photographic
assessment
,
histological
analysis
and
fluorescence
in
situ
hybridization
(
FISH
)
techniques
.
The
pressure
ulcer
group
(
n
 
=
 
12
)
was
created
using
a
compression
device
.
A
pressure
of
150
mmHg
for
8
h
,
with
a
total
of
three
cycles
of
compression-release
was
exerted
.
Evaluations
were
conducted
with
photographic
assessment
and
histological
analysis
.
Skin
grafts
in
the
control
group
took
successfully
,
as
shown
by
visual
assessment
,
FISH
techniques
and
histological
analysis
.
Pressure
ulcers
in
the
second
group
showed
full-thickness
skin
loss
with
damage
and
necrosis
of
all
the
epidermal
and
dermal
layers
(
ulcer
stage
III
)
in
all
cases
.
Complete
repair
occurred
after
40
days
.
An
inexpensive
,
reproducible
human
skin
pressure
ulcer
model
has
been
developed
.
This
novel
model
will
facilitate
the
development
of
new
clinically
relevant
therapeutic
strategies
that
can
be
tested
directly
on
human
skin
.
