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Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.
[neuralgic amyotrophy]
Peripheral
nerve
myelin
facilitates
rapid
impulse
conduction
and
normal
motor
and
sensory
functions
.
Many
aspects
of
myelin
biogenesis
,
glia-
axonal
interactions
,
and
nerve
homeostasis
are
poorly
understood
at
the
molecular
level
.
We
therefore
hypothesized
that
only
a
fraction
of
all
relevant
myelin
proteins
has
been
identified
so
far
.
Combining
gel-based
and
gel-free
proteomic
approaches
,
we
identified
545
proteins
in
purified
mouse
sciatic
nerve
myelin
,
including
36
previously
known
myelin
constituents
.
By
mass
spectrometric
quantification
,
the
predominant
P
0
,
periaxin
,
and
myelin
basic
protein
constitute
21
,
16
,
and
8
%
of
the
total
myelin
protein
,
respectively
,
suggesting
that
their
relative
abundance
was
previously
misestimated
due
to
technical
limitations
regarding
protein
separation
and
visualization
.
Focusing
on
tetraspan-transmembrane
proteins
,
we
validated
novel
myelin
constituents
using
immuno-based
methods
.
Bioinformatic
comparison
with
mRNA-abundance
profiles
allowed
the
categorization
in
functional
groups
coregulated
during
myelin
biogenesis
and
maturation
.
By
differential
myelin
proteome
analysis
,
we
found
that
the
abundance
of
septin
9
,
the
protein
affected
in
hereditary
neuralgic
amyotrophy
,
is
strongly
increased
in
a
novel
mouse
model
of
demyelinating
neuropathy
caused
by
the
loss
of
prion
protein
.
Finally
,
the
systematic
comparison
of
our
compendium
with
the
positions
of
human
disease
loci
allowed
us
to
identify
several
candidate
genes
for
hereditary
demyelinating
neuropathies
.
These
results
illustrate
how
the
integration
of
unbiased
proteome
,
transcriptome
,
and
genome
data
can
contribute
to
a
molecular
dissection
of
the
biogenesis
,
cell
biology
,
metabolism
,
and
pathology
of
myelin
.
Diseases
Validation
Diseases presenting
"axonal interactions"
symptom
neuralgic amyotrophy
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