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Antioxidants halt axonal degeneration in a mouse model of X-adrenoleukodystrophy.
[adrenomyeloneuropathy]
Axonal
degeneration
is
a
main
contributor
to
disability
in
progressive
neurodegenerative
diseases
in
which
oxidative
stress
is
often
identified
as
a
pathogenic
factor
.
We
aim
to
demonstrate
that
antioxidants
are
able
to
improve
axonal
degeneration
and
locomotor
deficits
in
a
mouse
model
of
X-
adrenoleukodystrophy
(
X-
ALD
)
.
X-
ALD
is
a
lethal
disease
caused
by
loss
of
function
of
the
ABCD
1
peroxisomal
transporter
of
very
long
chain
fatty
acids
(
VLCFA
)
.
The
mouse
model
for
X-
ALD
exhibits
a
late
onset
neurological
phenotype
with
locomotor
disability
and
axonal
degeneration
in
spinal
cord
resembling
the
most
common
phenotype
of
the
disease
,
adrenomyeloneuropathy
(
X-
AMN
)
.
Recently
,
we
identified
oxidative
damage
as
an
early
event
in
life
,
and
the
excess
of
VLCFA
as
a
generator
of
radical
oxygen
species
(
ROS
)
and
oxidative
damage
to
proteins
in
X-
ALD
.
Here
,
we
prove
the
capability
of
the
antioxidants
N-
acetyl-cysteine
,
α-lipoic
acid
,
and
α-tocopherol
to
scavenge
VLCFA-dependent
ROS
generation
in
vitro
.
Furthermore
,
in
a
preclinical
setting
,
the
cocktail
of
the
3
compounds
reversed
:
(
1
)
oxidative
stress
and
lesions
to
proteins
,
(
2
)
immunohistological
signs
of
axonal
degeneration
,
and
(
3
)
locomotor
impairment
in
bar
cross
and
treadmill
tests
.
We
have
established
a
direct
link
between
oxidative
stress
and
axonal
damage
in
a
mouse
model
of
neurodegenerative
disease
.
This
conceptual
proof
of
oxidative
stress
as
a
major
disease-driving
factor
in
X-
AMN
warrants
translation
into
clinical
trials
for
X-
AMN
,
and
invites
assessment
of
antioxidant
strategies
in
axonopathies
in
which
oxidative
damage
might
be
a
contributing
factor
.
Diseases
Validation
Diseases presenting
"degeneration in spinal cord"
symptom
adrenomyeloneuropathy
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