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Functional genomic analysis unravels a metabolic-inflammatory interplay in adrenoleukodystrophy.
[adrenomyeloneuropathy]
X-
linked
adrenoleukodystrophy
(
X-
ALD
)
is
an
inherited
disorder
characterized
by
axonopathy
and
demyelination
in
the
central
nervous
system
and
adrenal
insufficiency
.
Main
X-
ALD
phenotypes
are
:
(
i
)
an
adult
adrenomyeloneuropathy
(
AMN
)
with
axonopathy
in
spinal
cords
,
(
ii
)
cerebral
AMN
with
brain
demyelination
(
cAMN
)
and
(
iii
)
a
childhood
variant
,
cALD
,
characterized
by
severe
cerebral
demyelination
.
Loss
of
function
of
the
ABCD
1
peroxisomal
fatty
acid
transporter
and
subsequent
accumulation
of
very
-
long
-chain
fatty
acids
(
VLCFAs
)
are
the
common
culprits
to
all
forms
of
X-
ALD
,
an
aberrant
microglial
activation
accounts
for
the
cerebral
forms
,
whereas
inflammation
allegedly
plays
no
role
in
AMN
.
How
VLCFA
accumulation
leads
to
neurodegeneration
and
what
factors
account
for
the
dissimilar
clinical
outcomes
and
prognosis
of
X-
ALD
variants
remain
elusive
.
To
gain
insights
into
these
questions
,
we
undertook
a
transcriptomic
approach
followed
by
a
functional
-enrichment
analysis
in
spinal
cords
of
the
animal
model
of
AMN
,
the
Abcd
1
(
-
)
null
mice
,
and
in
normal-appearing
white
matter
of
cAMN
and
cALD
patients
.
We
report
that
the
mouse
model
shares
with
cAMN
and
cALD
a
common
signature
comprising
dysregulation
of
oxidative
phosphorylation
,
adipocytokine
and
insulin
signaling
pathways
,
and
protein
synthesis
.
Functional
validation
by
quantitative
polymerase
chain
reaction
,
western
blots
and
assays
in
spinal
cord
organotypic
cultures
confirmed
the
interplay
of
these
pathways
through
IkB
kinase
,
being
VLCFA
in
excess
a
causal
,
upstream
trigger
promoting
the
altered
signature
.
We
conclude
that
X-
ALD
is
,
in
all
its
variants
,
a
metabolic
/
inflammatory
syndrome
,
which
may
offer
new
targets
in
X-
ALD
therapeutics
.
Diseases
Validation
Diseases presenting
"neurodegeneration"
symptom
adrenomyeloneuropathy
alexander disease
cadasil
canavan disease
classical phenylketonuria
fabry disease
gm1 gangliosidosis
hereditary cerebral hemorrhage with amyloidosis
krabbe disease
neonatal adrenoleukodystrophy
neuralgic amyotrophy
oculocutaneous albinism
pyruvate dehydrogenase deficiency
triple a syndrome
wolf-hirschhorn syndrome
x-linked adrenoleukodystrophy
zellweger syndrome
This symptom has already been validated