Diagnostic utility of aP2/FABP4 expression in soft tissue tumours.

[well-differentiated liposarcoma]

Adipocyte P 2 (aP 2 ), also known as fatty acid-binding protein 4 (FABP 4 ), is a fatty acid-binding protein found in the cytoplasm of cells of adipocyte differentiation . In this study , we examined a large number of soft tissue tumours with a commercial polyclonal anti-a P 2 /FABP 4 antibody and a newly developed mouse monoclonal antibody raised against this protein to determine the diagnostic utility of aP 2 /FABP 4 as a marker of tumours of adipose differentiation . A mouse monoclonal antibody , clone 175 d , was raised against a mixture of synthetic peptides corresponding to the amino acid sequence of residues 10 -28 and 121 -132 of the human aP 2 /FABP 4 protein . Antigen expression with polyclonal and monoclonal antibodies was immunohistochemically determined in paraffin sections of normal adipose tissue and a wide range of benign and malignant primary soft tissue tumours (n = 200 ). aP 2 /FABP 4 was expressed around the cytoplasmic lipid vacuole in white and brown fat cells in benign lipomas and hibernomas . Immature fat cells and lipoblasts in spindle cell /pleomorphic lipoma , atypical lipomatous tumour /well-differentiated liposarcoma , myxoid /round cell liposarcoma and pleomorphic liposarcoma also reacted strongly for aP 2 /FABP 4 . No specific staining was seen in non-adipose benign and malignant mesenchymal and non-mesenchymal tumours . aP 2 /FABP 4 is expressed by mature and immature fat cells and is a marker of tumours of adipose differentiation . Immunophenotypic aP 2 /FABP 4 expression is useful in identifying lipoblasts , and immunohistochemistry with polyclonal /monoclonal anti-a P 2 /FABP 4 antibodies should be useful in distinguishing liposarcoma from other malignancies , particularly round cell , myxoid and pleomorphic soft tissue sarcomas .

Diseases
Validation

Diseases presenting "large number" symptom

acute rheumatic fever

adrenal incidentaloma

allergic bronchopulmonary aspergillosis

canavan disease

coats disease

cowden syndrome

dedifferentiated liposarcoma

dracunculiasis

epidermolysis bullosa simplex

fabry disease

familial mediterranean fever

gm1 gangliosidosis

heparin-induced thrombocytopenia

hereditary cerebral hemorrhage with amyloidosis

hirschsprung disease

kindler syndrome

legionellosis

malignant atrophic papulosis

neuralgic amyotrophy

phenylketonuria

pleomorphic liposarcoma

primary effusion lymphoma

scrub typhus

severe combined immunodeficiency

triple a syndrome

waldenström macroglobulinemia

well-differentiated liposarcoma

wiskott-aldrich syndrome

wolf-hirschhorn syndrome

x-linked adrenoleukodystrophy

zellweger syndrome

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