Diagnostic utility of aP2/FABP4 expression in soft tissue tumours.

[well-differentiated liposarcoma]

Adipocyte P 2 (aP 2 ), also known as fatty acid-binding protein 4 (FABP 4 ), is a fatty acid-binding protein found in the cytoplasm of cells of adipocyte differentiation . In this study , we examined a large number of soft tissue tumours with a commercial polyclonal anti-a P 2 /FABP 4 antibody and a newly developed mouse monoclonal antibody raised against this protein to determine the diagnostic utility of aP 2 /FABP 4 as a marker of tumours of adipose differentiation . A mouse monoclonal antibody , clone 175 d , was raised against a mixture of synthetic peptides corresponding to the amino acid sequence of residues 10 -28 and 121 -132 of the human aP 2 /FABP 4 protein . Antigen expression with polyclonal and monoclonal antibodies was immunohistochemically determined in paraffin sections of normal adipose tissue and a wide range of benign and malignant primary soft tissue tumours (n = 200 ). aP 2 /FABP 4 was expressed around the cytoplasmic lipid vacuole in white and brown fat cells in benign lipomas and hibernomas . Immature fat cells and lipoblasts in spindle cell /pleomorphic lipoma , atypical lipomatous tumour /well-differentiated liposarcoma , myxoid /round cell liposarcoma and pleomorphic liposarcoma also reacted strongly for aP 2 /FABP 4 . No specific staining was seen in non-adipose benign and malignant mesenchymal and non-mesenchymal tumours . aP 2 /FABP 4 is expressed by mature and immature fat cells and is a marker of tumours of adipose differentiation . Immunophenotypic aP 2 /FABP 4 expression is useful in identifying lipoblasts , and immunohistochemistry with polyclonal /monoclonal anti-a P 2 /FABP 4 antibodies should be useful in distinguishing liposarcoma from other malignancies , particularly round cell , myxoid and pleomorphic soft tissue sarcomas .

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