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Immuno-fluorescence based Vi capsular polysaccharide detection for specific recognition of Salmonella enterica serovar Typhi in clinical samples.
[typhoid]
Typhoid
fever
is
a
life
threatening
bacterial
infection
that
remains
a
major
global
health
concern
.
This
continued
high
burden
associated
with
significant
morbidity
and
mortality
rate
demands
specific
and
rapid
detection
technique
.
This
work
reports
a
new
sandwich
type
fluorescence
immunoassay
format
using
polymyxin
B
,
a
cationic
receptor
molecule
,
as
a
binder
agent
while
anti-
Vi
antibody
served
as
the
capturing
agent
for
specifically
detecting
Salmonella
enterica
serovar
Typhi
.
Anti-
Vi
IgG
antibody
raised
against
Vi
-
BSA
conjugate
revealed
affinity
of
7
.
779
nM
(
-
1
)
signifying
immunodominancy
of
O-
acetyls
groups
in
Vi
polysaccharide
.
The
detection
limit
of
the
developed
assay
was
around
10
(
1
)
cellsmL
(
-
1
)
of
Vi
expressing
Salmonella
enterica
serovar
Typhi
with
a
correlation
coefficient
(
R
(
2
)
)
equal
to
0
.
97
.
Positive
response
obtained
for
all
the
tested
serovar
Typhi
clinical
isolates
as
well
as
the
pathogen
spiked
blood
samples
recommended
specificity
and
accuracy
of
Vi
antigen
as
a
biomarker
during
typhoid
fever
.
The
intra-
and
inter-assay
precision
with
Vi
spiked
samples
were
satisfactory
revealing
coefficient
of
variance
(
CV
%
)
with
a
mean
of
4
.
05
%
and
5
.
97
%
respectively
.
This
may
be
the
novel
attempt
and
constructive
report
on
the
fluorescence
based
detection
of
Vi
antigen
of
serovar
Typhi
in
the
epidemic
as
well
as
pandemic
outbreaks
.
Diseases
Validation
Diseases presenting
"fever"
symptom
22q11.2 deletion syndrome
acute rheumatic fever
alexander disease
allergic bronchopulmonary aspergillosis
canavan disease
carcinoma of the gallbladder
child syndrome
congenital toxoplasmosis
cushing syndrome
cystinuria
dracunculiasis
erdheim-chester disease
esophageal adenocarcinoma
esophageal carcinoma
familial mediterranean fever
focal myositis
hodgkin lymphoma, classical
lamellar ichthyosis
legionellosis
locked-in syndrome
malignant atrophic papulosis
neonatal adrenoleukodystrophy
neuralgic amyotrophy
oculocutaneous albinism
papillon-lefèvre syndrome
pyomyositis
pyruvate dehydrogenase deficiency
scrub typhus
severe combined immunodeficiency
sneddon syndrome
systemic capillary leak syndrome
triple a syndrome
typhoid
waldenström macroglobulinemia
wolf-hirschhorn syndrome
This symptom has already been validated