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Immuno-fluorescence based Vi capsular polysaccharide detection for specific recognition of Salmonella enterica serovar Typhi in clinical samples.
[typhoid]
Typhoid
fever
is
a
life
threatening
bacterial
infection
that
remains
a
major
global
health
concern
.
This
continued
high
burden
associated
with
significant
morbidity
and
mortality
rate
demands
specific
and
rapid
detection
technique
.
This
work
reports
a
new
sandwich
type
fluorescence
immunoassay
format
using
polymyxin
B
,
a
cationic
receptor
molecule
,
as
a
binder
agent
while
anti-
Vi
antibody
served
as
the
capturing
agent
for
specifically
detecting
Salmonella
enterica
serovar
Typhi
.
Anti-
Vi
IgG
antibody
raised
against
Vi
-
BSA
conjugate
revealed
affinity
of
7
.
779
nM
(
-
1
)
signifying
immunodominancy
of
O-
acetyls
groups
in
Vi
polysaccharide
.
The
detection
limit
of
the
developed
assay
was
around
10
(
1
)
cellsmL
(
-
1
)
of
Vi
expressing
Salmonella
enterica
serovar
Typhi
with
a
correlation
coefficient
(
R
(
2
)
)
equal
to
0
.
97
.
Positive
response
obtained
for
all
the
tested
serovar
Typhi
clinical
isolates
as
well
as
the
pathogen
spiked
blood
samples
recommended
specificity
and
accuracy
of
Vi
antigen
as
a
biomarker
during
typhoid
fever
.
The
intra-
and
inter-assay
precision
with
Vi
spiked
samples
were
satisfactory
revealing
coefficient
of
variance
(
CV
%
)
with
a
mean
of
4
.
05
%
and
5
.
97
%
respectively
.
This
may
be
the
novel
attempt
and
constructive
report
on
the
fluorescence
based
detection
of
Vi
antigen
of
serovar
Typhi
in
the
epidemic
as
well
as
pandemic
outbreaks
.
