Rare Diseases Symptoms Automatic Extraction
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Evaluation of T and B lymphocyte function in clinical practice using a flow cytometry based proliferation assay.
[severe combined immunodeficiency]
The
golden
standard
for
functional
evaluation
of
immunodeficiencies
is
the
incorporation
of
[
(
3
)
H
]
-
thymidine
in
a
proliferation
assay
stimulated
with
mitogens
.
Recently
developed
whole
blood
proliferation
assays
have
the
advantage
of
parallel
lymphocyte
lineage
analysis
and
in
addition
provide
a
non-radioactive
alternative
.
Here
we
evaluate
the
Flow-cytometric
Assay
for
Specific
Cell-mediated
Immune-response
in
Activated
whole
blood
(
FASCIA
)
in
a
comparison
with
[
(
3
)
H
]
-
thymidine
incorporation
in
four
patients
with
severe
combined
immunodeficiency
.
The
threshold
for
the
minimum
number
of
lymphocytes
required
for
reliable
responses
in
FASCIA
is
determined
together
with
reference
values
from
100
healthy
donors
when
stimulated
with
mitogens
as
well
as
antigen
specific
stimuli
.
Finally
,
responses
against
PWM
and
SEA
+
SEB
stimuli
are
conducted
with
clinically
relevant
immunomodulatory
compounds
.
We
conclude
that
FASCIA
is
a
rapid
,
stable
and
sensitive
functional
whole
blood
assay
that
requires
small
amounts
of
whole
blood
that
can
be
used
for
reliable
assessment
of
lymphocyte
reactivity
in
patients
.
