Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Placental mesenchymal stem cells of fetal origin deposit epigenetic alterations during long-term culture under serum-free condition.
[severe combined immunodeficiency]
Objective
:
Fetal
placental
mesenchymal
stem
cells
(
fPMSCs
)
have
shown
promising
cell
therapy
potentials
.
However
,
their
genetic
and
epigenetic
stability
during
in
vitro
propagation
has
not
been
well
studied
.
We
thus
interrogated
the
methylation
alterations
and
tumorigenicity
of
fPMSCs
after
in
vitro
expansion
using
serum-free
medium
.
Research
design
and
methods
:
The
properties
of
fPMSCs
cultured
in
a
serum-free
medium
at
passage
3
and
passage
8
were
ascertained
by
determining
their
MSC
markers
,
proliferative
capacity
,
chromosomal
stability
,
activity
of
global
DNA
methyltransferases
and
methylation
profile
.
Their
potential
of
malignant
transformation
was
also
evaluated
in
a
severe
combined
immunodeficiency
(
SCID
)
murine
model
.
Results
:
The
fPMSCs
could
maintain
their
MSC
characteristics
but
quickly
reached
a
senescent
state
of
proliferation
during
in
vitro
expansion
.
246
genes
with
differential
DNA
methylation
of
promoters
were
identified
,
along
with
a
significantly
downregulated
global
DNA
methyltransferase
activity
.
The
genes
associated
with
aging
and
tumorigenesis
had
a
significantly
demethylated
tendency
over
in
vitro
propagation
.
However
,
the
deposition
of
epigenetic
alterations
did
not
translate
into
malignant
transformation
in
SCID
mice
.
Conclusion
:
The
fPMSCs
cultured
in
serum-free
medium
have
a
tendency
to
deposit
methylation
modifications
over
in
vitro
expansion
,
therefore
the
detection
of
genetic
and
/
or
epigenetic
alterations
is
necessary
for
fPMSCs
before
they
are
employed
for
clinical
uses
.
Diseases
Validation
Diseases presenting
"malignant transformation"
symptom
achondroplasia
adrenal incidentaloma
child syndrome
cholangiocarcinoma
cutaneous mastocytosis
epidermolysis bullosa simplex
esophageal adenocarcinoma
liposarcoma
oral submucous fibrosis
severe combined immunodeficiency
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom