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Mechanisms of expression of pyruvate dehydrogenase deficiency caused by an E1alpha subunit mutation.
[pyruvate dehydrogenase deficiency]
To
characterize
the
biochemical
mechanisms
of
expression
of
the
pyruvate
dehydrogenase
(
PDH
)
E
1
alpha
subunit
exon
10
R
302
C
missense
mutation
.
Mutations
in
the
X-
linked
E
1
alpha
subunit
gene
are
responsible
for
most
cases
of
PDH
deficiency
,
an
important
cause
of
neurodevelopmental
defects
and
neurodegeneration
with
primary
lactic
acidemia
.
Although
the
disease
shows
extreme
allelic
heterogeneity
,
the
R
302
C
mutation
has
been
defined
in
several
unrelated
cases
.
Cell
lines
expressing
selectively
either
the
mutant
or
wild-
type
E
1
alpha
alleles
against
identical
genetic
backgrounds
were
generated
from
the
fibroblasts
of
a
female
heterozygous
for
the
R
302
C
mutation
.
Enzyme
activity
,
mRNA
,
polypeptide
expression
,
and
turnover
were
studied
in
each
.
The
residual
PDH
activity
was
below
measurable
levels
in
the
cell
line
(
B
5
)
expressing
only
the
mutant
allele
and
normal
in
the
wild-
type
polypeptide
expressing
(
A
10
)
cell
line
,
confirming
that
the
R
302
C
mutation
alone
is
sufficient
to
cause
a
severe
PDH
deficiency
.
The
mutant
polypeptide
was
less
stable
than
the
wild-
type
polypeptide
,
but
the
steady-
state
level
of
the
mutant
E
1
alpha
protein
was
reduced
only
two
-
to
threefold
.
The
primary
mechanism
of
expression
of
the
R
302
C
mutation
must
be
limitation
of
catalytic
efficiency
.
We
speculate
that
catalysis
may
be
inhibited
in
the
mutant
polypeptide
because
conformational
changes
are
induced
near
serine
300
,
a
residue
that
is
particularly
important
as
a
regulatory
phosphorylation
site
in
the
wild-
type
polypeptide
.
Diseases
Validation
Diseases presenting
"the r302c mutation has been defined in several unrelated cases"
symptom
pyruvate dehydrogenase deficiency
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