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Histochemical analysis of polarizing colors of collagen using Picrosirius Red staining in oral submucous fibrosis.
[oral submucous fibrosis]
Oral
Submucous
fibrosis
(
OSMF
)
is
a
common
potentially
malignant
disease
,
which
is
characterized
by
generalized
fibrosis
of
the
oral
soft
tissues
.
It
is
due
to
disproportion
in
the
collagen
deposition
and
degradation
.
The
excess
deposition
of
collagen
results
in
juxta
epithelial
fibro
elastic
changes
and
epithelial
atrophy
.
Neoplastic
changes
in
the
epithelium
usually
precede
the
changes
in
the
connective
tissue
.
The
purpose
of
the
study
was
to
evaluate
the
changes
in
birefringence
of
collagen
using
picrosirius
stain
and
compare
with
haematoxylin
and
eosin
stained
section
.
The
study
was
conducted
on
50
subjects
,
which
included
45
patients
diagnosed
with
different
functional
and
histopathological
grades
of
OSMF
and
5
in
control
group
.
Picrosirius
red
stain
was
used
to
analyze
collagen
both
histopathologically
and
qualitatively
using
polarizing
microscopy
.
Chi
-square
test
at
p
value
<
0
.
05
was
used
to
analyze
the
results
and
the
significance
was
tabulated
.
Collagen
fibers
showed
mixed
birefringence
with
a
shift
in
polarizing
colour
from
yellow
to
red
-orange
in
lamina
propria
,
around
the
muscle
and
blood
vessels
,
which
was
correlating
with
the
conventional
H
&
E
stain
.
The
results
of
present
study
show
a
significant
change
in
birefringence
of
collagen
between
the
various
components
of
connective
tissue
.
This
change
in
birefringence
colors
and
arrangement
of
collagen
fibers
might
give
an
assumption
of
impending
neoplastic
change
in
OSMF
.
How
to
cite
the
article
:
Velidandla
S
,
Gaikwad
P
,
Ealla
KK
,
Bhorgonde
KD
,
Hunsingi
P
,
Kumar
A
.
Histochemical
analysis
of
polarizing
colors
of
collagen
using
Picrosirius
Red
staining
in
oral
submucous
fibrosis
.
J
Int
Oral
Health
2014
;
6
(
1
)
:
33
-
8
.
