Rare Diseases Symptoms Automatic Extraction
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A random Abstract
Our Project
Our Team
Molecular screening of lysyl oxidase G473A polymorphism in oral submucous fibrosis.
[oral submucous fibrosis]
To
investigate
the
presence
of
lysyl
oxidase
(
LOX
)
G
473
A
polymorphism
in
group
1
{
Oral
submucous
fibrosis
(
OSMF
)
patients
}
,
group
2
(
betel
quid
chewers
without
OSMF
)
and
group
3
(
healthy
individuals
)
.
A
total
of
60
patients
were
taken
for
the
study
,
which
included
20
OSMF
patients
(
group
1
)
,
20
betel
quid
chewers
without
OSMF
(
group
2
)
and
20
healthy
individuals
without
OSMF
and
betel
quid
chewing
habit
(
group
3
)
.
DNA
was
isolated
using
Qiagen
kit
.
The
isolated
DNA
was
quantified
using
spectroscopic
methods
.
Polymerase
chain
reaction
(
PCR
)
was
carried
out
at
annealing
temperature
of
67
(
o
)
C
.
PCR
amplification
was
checked
on
2
%
agarose
gel
.
Further
,
the
amplified
PCR
products
were
subjected
to
automated
DNA
sequencer
,
to
assess
LOX
G
473
A
polymorphism
.
The
gene
sequence
data
generated
from
the
automated
DNA
sequencer
was
received
as
colored
electropherograms
.
These
gene
-sequencing
results
did
not
show
LOX
G
473
A
polymorphism
in
any
of
the
3
groups
.
In
our
study
,
gene
-sequencing
results
did
not
show
LOX
G
473
A
polymorphism
in
OSMF
patients
.
Since
only
one
study
in
the
literature
has
shown
the
association
of
LOX
gene
polymorphism
and
OSMF
patients
,
we
conclude
that
further
studies
are
required
to
unveil
the
role
of
LOX
gene
polymorphism
in
OSMF
.
