Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis.
[oculocutaneous albinism]
In
the
past
decade
,
Xenopus
tropicalis
has
emerged
as
a
powerful
new
amphibian
genetic
model
system
,
which
offers
all
of
the
experimental
advantages
of
its
larger
cousin
,
Xenopus
laevis
.
Here
we
investigated
the
efficiency
of
transcription
activator-like
effector
nucleases
(
TALENs
)
for
generating
targeted
mutations
in
endogenous
genes
in
X
.
tropicalis
.
For
our
analysis
we
targeted
the
tyrosinase
(
oculocutaneous
albinism
IA
)
(
tyr
)
gene
,
which
is
required
for
the
production
of
skin
pigments
,
such
as
melanin
.
We
injected
mRNA
encoding
TALENs
targeting
the
first
exon
of
the
tyr
gene
into
two
-cell-
stage
embryos
.
Surprisingly
,
we
found
that
over
90
%
of
the
founder
animals
developed
either
partial
or
full
albinism
,
suggesting
that
the
TALENs
induced
bi
-allelic
mutations
in
the
tyr
gene
at
very
high
frequency
in
the
F
0
animals
.
Furthermore
,
mutations
tyr
gene
were
efficiently
transmitted
into
the
F
1
progeny
,
as
evidenced
by
the
generation
of
albino
offspring
.
These
findings
have
far
reaching
implications
in
our
quest
to
develop
efficient
reverse
genetic
approaches
in
this
emerging
amphibian
model
.
Diseases
Validation
Diseases presenting
"suggesting that the talens induced bi-allelic mutations in the tyr gene at very high frequency in the f0 animals"
symptom
oculocutaneous albinism
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom