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A random Abstract
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Our Team
Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis.
[oculocutaneous albinism]
In
the
past
decade
,
Xenopus
tropicalis
has
emerged
as
a
powerful
new
amphibian
genetic
model
system
,
which
offers
all
of
the
experimental
advantages
of
its
larger
cousin
,
Xenopus
laevis
.
Here
we
investigated
the
efficiency
of
transcription
activator-like
effector
nucleases
(
TALENs
)
for
generating
targeted
mutations
in
endogenous
genes
in
X
.
tropicalis
.
For
our
analysis
we
targeted
the
tyrosinase
(
oculocutaneous
albinism
IA
)
(
tyr
)
gene
,
which
is
required
for
the
production
of
skin
pigments
,
such
as
melanin
.
We
injected
mRNA
encoding
TALENs
targeting
the
first
exon
of
the
tyr
gene
into
two
-cell-
stage
embryos
.
Surprisingly
,
we
found
that
over
90
%
of
the
founder
animals
developed
either
partial
or
full
albinism
,
suggesting
that
the
TALENs
induced
bi
-allelic
mutations
in
the
tyr
gene
at
very
high
frequency
in
the
F
0
animals
.
Furthermore
,
mutations
tyr
gene
were
efficiently
transmitted
into
the
F
1
progeny
,
as
evidenced
by
the
generation
of
albino
offspring
.
These
findings
have
far
reaching
implications
in
our
quest
to
develop
efficient
reverse
genetic
approaches
in
this
emerging
amphibian
model
.