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In vitro derivation of macrophage from guinea pig bone marrow with human M-CSF.
[legionellosis]
The
guinea
pig
has
a
storied
history
as
a
model
in
the
study
of
infectious
disease
and
immunology
.
Because
of
reproducibility
of
data
and
availability
of
various
reagents
,
inbred
mice
have
since
supplanted
the
guinea
pig
as
the
animal
model
-of-choice
in
these
fields
.
However
,
several
clinically-significant
microorganisms
do
not
cause
the
same
pathology
in
mice
,
or
mice
may
not
be
susceptible
to
these
infections
.
These
demonstrate
the
utility
of
other
animal
models
-
either
as
the
primary
method
to
study
a
particular
infection
,
or
to
confirm
or
refute
findings
in
the
mouse
before
translating
basic
science
into
clinical
practice
.
The
mononuclear
phagocyte
,
or
macrophage
(
Mφ
)
,
plays
a
key
role
in
antigen
presentation
and
the
pathogenesis
of
intracellular
bacteria
,
such
as
Mycobacterium
tuberculosis
and
Legionella
pneumophila
.
Because
of
variable
yield
and
difficult
extraction
from
tissue
,
the
preferred
method
of
producing
Mφ
for
in
vitro
studies
is
to
expand
murine
bone
marrow
(
BM
)
precursors
with
mouse
macrophage
colony-stimulating
factor
(
M-CSF
)
.
This
has
not
been
shown
in
the
guinea
pig
.
Here
,
we
report
the
empiric
observation
that
human
M-CSF
-
but
not
mouse
M-CSF
,
nor
human
granulocyte
/
macrophage
colony-stimulating
factor
-
can
be
used
to
induce
BM
precursor
differentiation
into
bonafide
Mφ
.
The
differentiated
cells
appeared
as
enlarged
adherent
cells
,
capable
of
both
pinocytosis
and
large
particle
phagocytosis
.
Furthermore
,
we
showed
that
these
guinea
pig
BM-derived
Mφ
,
similar
to
human
monocyte
/
Mφ
lines
but
unlike
most
murine
BM
Mφ
,
support
growth
of
wild
type
L
.
pneumophila
.
This
method
may
prove
useful
for
in
vitro
studies
of
Mφ
in
the
guinea
pig
,
as
well
as
in
the
translation
of
results
found
using
mouse
BM-derived
Mφ
towards
studies
in
human
immunology
and
infectious
disease
.
Diseases
Validation
Diseases presenting
"primary method"
symptom
legionellosis
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