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Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trials.
[legionellosis]
Legionellosis
is
an
uncommon
form
of
pneumonia
.
After
a
clinical
encounter
,
the
necessary
antibiotic
treatment
is
available
if
the
diagnosis
is
made
early
in
the
illness
.
Before
the
clinical
encounter
,
early
detection
of
the
main
pathogen
involved
,
Legionella
pneumophila
,
in
hazardous
environments
is
important
in
preventing
infectious
levels
of
this
bacterium
.
In
this
study
a
qualitative
test
based
on
combined
magnetic
immunocapture
and
enzyme-immunoassay
for
the
fast
detection
of
Legionella
pneumophila
in
water
samples
was
compared
with
the
standard
method
,
in
both
comparative
and
collaborative
trials
.
The
test
was
based
on
the
use
of
anti-
Legionella
pneumophila
antibodies
immobilized
on
magnetic
microspheres
.
The
final
protocol
included
concentration
by
filtration
,
resuspension
and
immunomagnetic
capture
.
The
whole
assay
took
less
than
1
hour
to
complete
.
A
comparative
trial
was
performed
against
the
standard
culture
method
(
ISO
11731
)
on
both
artificially
and
naturally
contaminated
water
samples
,
for
two
matrices
:
chlorinated
tap
water
and
cooling
tower
water
.
Performance
characteristics
of
the
test
used
as
screening
with
culture
confirmation
resulted
in
sensitivity
,
specificity
,
false
positive
,
false
negative
,
and
efficiency
of
96
.
6
%
,
100
%
,
0
%
,
3
.
4
%
,
and
97
.
8
%
,
respectively
.
The
detection
limit
at
the
level
under
which
the
false
negative
rate
increases
to
50
%
(
LOD
50
)
was
93
colony
forming
units
(
CFU
)
in
the
volume
examined
for
both
tested
matrices
.
The
collaborative
trial
included
twelve
laboratories
.
Water
samples
spiked
with
certified
reference
materials
were
tested
.
In
this
study
the
coincidence
level
between
the
two
methods
was
95
.
8
%
.
Results
demonstrate
the
applicability
of
this
immunosensing
technique
to
the
rapid
,
simple
,
and
efficient
detection
of
Legionella
pneumophila
in
water
samples
.
This
test
is
not
based
on
microbial
growth
,
so
it
could
be
used
as
a
rapid
screening
technique
for
the
detection
of
L
.
pneumophila
in
waters
,
maintaining
the
performance
of
conventional
culture
for
isolation
of
the
pathogen
and
related
studies
.
Diseases
Validation
Diseases presenting
"certified reference materials"
symptom
legionellosis
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