Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
VBNC Legionella pneumophila cells are still able to produce virulence proteins.
[legionellosis]
Legionella
pneumophila
is
the
agent
responsible
for
legionellosis
.
Numerous
bacteria
,
including
L
.
pneumophila
,
can
enter
into
a
viable
but
not
culturable
(
VBNC
)
state
under
unfavorable
environmental
conditions
.
In
this
state
,
cells
are
unable
to
form
colonies
on
standard
medium
but
are
still
alive
.
Here
we
show
that
VBNC
L
.
pneumophila
cells
,
obtained
by
monochloramine
treatment
,
were
still
able
to
synthesize
proteins
,
some
of
which
are
involved
in
virulence
.
Protein
synthesis
was
measured
using
(
35
)
S-
labeling
and
the
proteomes
of
VBNC
and
culturable
cells
then
compared
.
This
analysis
allowed
the
identification
of
nine
proteins
that
were
accumulated
in
the
VBNC
state
.
Among
them
,
four
were
involved
in
virulence
,
i
.
e
.
,
the
macrophage
infectivity
potentiator
protein
,
the
hypothetical
protein
lpl
2247
,
the
ClpP
protease
proteolytic
subunit
and
the
27
kDa
outer
membrane
protein
.
Others
,
i
.
e
.
,
the
enoyl
reductase
,
the
electron
transfer
flavoprotein
(
alpha
and
beta
subunits
)
,
the
50
S
ribosomal
proteins
(
L
1
and
L
25
)
are
involved
in
metabolic
and
energy
production
pathways
.
However
,
resuscitation
experiments
performed
with
Acanthamoeba
castellanii
failed
,
suggesting
that
the
accumulation
of
virulence
factors
by
VBNC
cells
is
not
sufficient
to
maintain
their
virulence
.
Diseases
Validation
Diseases presenting
"unfavorable environmental conditions"
symptom
legionellosis
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom