Rare Diseases Symptoms Automatic Extraction
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Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR.
[legionellosis]
Legionella
pneumophila
is
the
primary
cause
of
the
legionellosis
diseases
(
90
%
)
(
Yu
et
al
.
in
J
Infect
Dis
186
:
127
-
128
,
2002
;
Doleans
et
al
.
in
J
Clin
Microbiol
42
:
458
-
460
,
2004
;
Den
Boer
et
al
.
in
Clin
Microbiol
Infect
14
:
459
-
466
,
2008
)
.
In
this
study
,
methodologies
based
on
molecular
biology
were
developed
in
order
to
provide
a
quick
diagnosis
of
the
bacterial
presence
in
water
samples
of
Spain
.
Multiplex
real-time
polymerase
chain
reaction
assays
were
realized
to
target
the
16
S
rRNA
and
macrophage
infectivity
potentiator
(
mip
)
genes
of
,
respectively
,
Legionella
spp
.
and
L
.
pneumophila
including
in
the
design
of
an
internal
control
.
The
results
obtained
by
the
culture
and
the
gene
amplification
methods
agreed
in
94
.
44
%
for
the
16
S
rRNA
gene
,
and
a
concordance
of
66
.
67
%
of
the
cases
was
obtained
for
the
mip
gene
.
