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A random Abstract
Our Project
Our Team
LC-MS/MS analysis of epoxyalcohols and epoxides of arachidonic acid and their oxygenation by recombinant CYP4F8 and CYP4F22.
[lamellar ichthyosis]
CYP
4
F
2
2
and
CYP
4
F
8
are
expressed
in
epidermis
,
and
mutations
of
CYP
4
F
2
2
are
associated
with
lamellar
ichthyosis
.
Epoxyalcohols
(
HEETs
)
and
epoxides
(
EETs
)
of
20
:
4
n-
6
appear
to
be
important
for
the
water
permeability
barrier
of
skin
.
Our
aim
was
to
study
the
MS
/
MS
spectra
and
fragmentation
of
these
compounds
and
to
determine
whether
they
were
oxidized
by
CYP
4
F
2
2
or
CYP
4
F
8
expressed
in
yeast
.
HEETs
were
prepared
from
15
-
hydroperoxyeicosatetraenoic
acid
(
15
-
HPETE
)
,
12
-
HPETE
,
and
their
[
(
2
)
H
(
8
)
]
labeled
isotopomers
,
and
separated
by
normal
phase-
HPLC
with
MS
/
MS
analysis
.
CYP
4
F
2
2
oxygenated
20
:
4
n-
6
at
C-
18
,
whereas
metabolites
of
HEETs
could
not
be
identified
.
CYP
4
F
8
formed
omega
3
hydroxy
metabolites
of
HEETs
derived
from
12
R-HPETE
with
11
,
12
-
epoxy-
10
-
hydroxy
configuration
,
but
not
HEETs
derived
from
15
S-HPETE
.
8
,
9
-
EET
and
11
,
12
-
EET
were
also
subject
to
omega
3
hydroxylation
by
CYP
4
F
8
.
We
conclude
that
CYP
4
F
8
and
CYP
4
F
2
2
oxidize
20
:
4
n-
6
and
that
CYP
4
F
8
selectively
oxidizes
8
,
9
-
EET
,
11
,
12
-
EET
,
and
10
,
11
R
,
12
R-HEET
at
the
omega
3
position
.
Diseases
Validation
Diseases presenting
"ichthyosis"
symptom
child syndrome
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
harlequin ichthyosis
hirschsprung disease
junctional epidermolysis bullosa
kallmann syndrome
lamellar ichthyosis
This symptom has already been validated