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A random Abstract
Our Project
Our Team
A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.
[hodgkin lymphoma, classical]
We
have
recently
demonstrated
that
classical
Hodgkin
lymphoma
(
CHL
)
can
be
immunophenotyped
by
flow
cytometry
(
FC
)
,
thus
obviating
the
need
for
immunohistochemistry
in
many
cases
.
The
previously
described
nine
-color
assay
,
however
,
can
not
be
used
by
laboratories
that
do
not
have
access
to
a
nine
-
or
ten
-color
flow
cytometer
.
Therefore
,
a
six
-color
FC
tube
was
designed
employing
the
following
combination
:
CD
6
4
-
FITC
/
CD
30
-
PE
/
CD
40
-
PeCy
5
.
5
/
CD
2
0
-
PECy
7
/
CD
9
5
-
APC
/
CD
3
-
APC
-H
7
.
To
validate
this
assay
,
we
analyzed
408
tissue
specimens
(
including
55
CHL
cases
,
26
of
which
had
been
previously
cryopreserved
)
.
Specimen
inclusion
criteria
included
the
identification
of
an
abnormal
population
by
FC
or
(
if
no
abnormal
population
was
identified
)
greater
than
50
,
000
viable
events
and
specimen
age
less
than
4
days
.
All
FC
studies
were
examined
blinded
to
any
clinical
,
laboratory
,
or
histologic
information
.
The
diagnostic
sensitivity
and
specificity
of
the
six
-color
FC
assay
was
85
.
4
%
and
99
.
7
%
,
respectively
.
Taken
together
,
these
results
suggest
that
the
six
-color
FC
assay
has
acceptable
sensitivity
and
specificity
for
clinical
use
,
allowing
more
FC
laboratories
to
immunophenotype
CHL
by
this
method
.
Diseases
Validation
Diseases presenting
"hodgkin lymphoma"
symptom
esophageal adenocarcinoma
hodgkin lymphoma, classical
monosomy 21
primary effusion lymphoma
severe combined immunodeficiency
systemic capillary leak syndrome
waldenström macroglobulinemia
This symptom has already been validated