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A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes.
[hodgkin lymphoma, classical]
We
have
recently
demonstrated
that
classical
Hodgkin
lymphoma
(
CHL
)
can
be
immunophenotyped
by
flow
cytometry
(
FC
)
,
thus
obviating
the
need
for
immunohistochemistry
in
many
cases
.
The
previously
described
nine
-color
assay
,
however
,
can
not
be
used
by
laboratories
that
do
not
have
access
to
a
nine
-
or
ten
-color
flow
cytometer
.
Therefore
,
a
six
-color
FC
tube
was
designed
employing
the
following
combination
:
CD
6
4
-
FITC
/
CD
30
-
PE
/
CD
40
-
PeCy
5
.
5
/
CD
2
0
-
PECy
7
/
CD
9
5
-
APC
/
CD
3
-
APC
-H
7
.
To
validate
this
assay
,
we
analyzed
408
tissue
specimens
(
including
55
CHL
cases
,
26
of
which
had
been
previously
cryopreserved
)
.
Specimen
inclusion
criteria
included
the
identification
of
an
abnormal
population
by
FC
or
(
if
no
abnormal
population
was
identified
)
greater
than
50
,
000
viable
events
and
specimen
age
less
than
4
days
.
All
FC
studies
were
examined
blinded
to
any
clinical
,
laboratory
,
or
histologic
information
.
The
diagnostic
sensitivity
and
specificity
of
the
six
-color
FC
assay
was
85
.
4
%
and
99
.
7
%
,
respectively
.
Taken
together
,
these
results
suggest
that
the
six
-color
FC
assay
has
acceptable
sensitivity
and
specificity
for
clinical
use
,
allowing
more
FC
laboratories
to
immunophenotype
CHL
by
this
method
.
Diseases
Validation
Diseases presenting
"abnormal population"
symptom
hodgkin lymphoma, classical
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