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Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation.
[gm1 gangliosidosis]
Repression
of
human
papillomavirus
(
HPV
)
E
6
and
E
7
oncogenes
in
established
cervical
carcinoma
cell
lines
causes
senescence
due
to
reactivation
of
cellular
tumor
suppressor
pathways
.
Here
,
we
determined
whether
ongoing
expression
of
HPV
16
or
HPV
18
oncogenes
is
required
for
the
proliferation
of
primary
human
cervical
carcinoma
cells
in
serum-free
conditions
at
low
passage
number
after
isolation
from
patients
.
We
used
an
SV
40
viral
vector
expressing
the
bovine
papillomavirus
E
2
protein
to
repress
E
6
and
E
7
in
these
cells
.
To
enable
efficient
SV
40
infection
and
E
2
gene
delivery
,
we
first
incubated
the
primary
cervical
cancer
cells
with
the
ganglioside
GM
1
,
a
cell-surface
receptor
for
SV
40
that
is
limiting
in
these
cells
.
Repression
of
HPV
in
primary
cervical
carcinoma
cells
caused
them
to
undergo
senescence
,
but
the
E
2
protein
had
little
effect
on
HPV-negative
primary
cells
.
These
data
suggest
that
E
6
and
E
7
dependence
is
an
inherent
property
of
human
cervical
cancer
cells
.
