Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities.
[esophageal squamous cell carcinoma]
Eukaryotic
DNA
is
packaged
into
a
chromatin
with
the
help
of
four
core
histones
(
H
2
A
,
H
2
B
,
H
3
,
and
H
4
)
.
Diverse
histone
post-translational
modifications
(
PTMs
)
are
hence
involved
in
the
regulation
of
gene
transcription
.
However
,
how
this
regulation
does
work
is
still
poorly
understood
and
lacks
details
.
Here
we
used
the
mass
spectrometry-based
proteomics
approach
to
perform
a
comparative
analysis
of
histone
marks
at
a
global
level
in
two
phenotypes
of
esophageal
squamous
cell
carcinoma
(
ESCC
)
with
different
invasiveness
.
We
obtained
a
comprehensive
profiling
of
histone
H
3
and
H
4
PTMs
including
lysine
methylation
,
acetylation
and
novel
butyrylation
.
The
correlation
between
histone
marks
and
cancer
invasive
capabilities
was
further
characterized
and
one
distinguishable
PTM
,
H
4
K
79
me
2
was
discovered
and
verified
in
this
study
.
Immunohistochemistry
analysis
suggests
that
abnormal
level
of
H
4
K
79
me
2
may
be
related
to
poor
survival
of
ESCC
patients
.
Our
results
enrich
the
dataset
of
the
feature
pattern
of
global
histone
PTMs
in
ESCC
cell
lines
.
C
ore
histone
proteins
,
decorated
by
multiple
biological
significant
protein
post-translational
modifications
(
PTMs
)
such
as
lysine
acetylation
and
lysine
methylation
,
are
considered
to
regulate
gene
transcription
and
be
associated
with
the
development
of
cancer
.
Recent
studies
have
further
shown
that
global
level
of
histone
modifications
is
the
potential
hallmark
of
cancer
to
predict
the
clinical
outcomes
of
human
cancers
.
However
,
the
regulation
mechanism
is
largely
unknown
.
Here
we
used
the
mass
spectrometry
based
proteomics
coupled
with
stable
isotope
labeling
with
amino
acids
in
cell
culture
(
SILAC
)
to
characterize
the
global
levels
of
histone
marks
in
two
phenotypes
of
esophageal
squamous
cell
carcinoma
(
ESCC
)
cell
lines
with
different
invasive
capabilities
.
To
the
best
of
our
knowledge
,
it
is
the
first
report
about
the
comparative
analysis
for
histone
marks
of
the
different
invasive
ESCC
cell
lines
.
A
significantly
differential
level
of
histone
modification
,
H
4
K
79
me
2
,
was
determined
and
verified
.
Immunohistochemistry
analysis
further
suggests
that
abnormal
level
of
H
4
K
79
me
2
may
be
related
to
poor
survival
of
ESCC
patients
.
Our
results
could
contribute
to
understanding
the
different
expressions
of
global
histone
PTMs
in
different
invasive
ESCC
cell
lines
.
Diseases
Validation
Diseases presenting
"different invasive capabilities"
symptom
esophageal squamous cell carcinoma
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom