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Anti-tumor effects of flavonoids from the ethnic medicine Docynia delavayi (Franch.) Schneid. and its possible mechanism.
[esophageal carcinoma]
This
study
investigated
the
active
components
and
the
anti-
tumor
efficacy
and
mechanisms
of
the
flavonoids
from
Docynia
delavayi
(
Franch
.
)
Schneid
.
(
DDS
)
.
MTT
assay
was
used
to
examine
the
growth
inhibitory
effects
of
the
four
flavonoids
,
including
chrysin
,
quercetin
,
naringenin
,
and
avicularin
that
were
isolated
from
the
rhizome
of
DDS
,
on
human
hematomas
cell
(
HepG
2
)
,
esophageal
carcinoma
cell
(
EC
109
)
,
human
cervical
adenocarcinoma
cell
(
Hela
)
,
human
colon
adenocarcinoma
cell
(
SW
480
)
,
and
African
green
monkey
kidney
cell
(
Vero
cells
)
.
The
anti-
tumor
mechanism
of
chrysin
on
HepG
2
was
further
investigated
by
the
methods
of
fluorescence
staining
,
flow
cytometry
,
and
immunoblotting
.
The
results
showed
that
the
inhibitory
activity
of
chrysin
was
much
stronger
than
the
other
three
flavonoids
on
HepG
2
,
EC
109
,
Hela
,
and
SW
480
cells
for
48
h
treatment
in
vitro
.
Moreover
,
no
inhibiting
effect
of
chrysin
on
the
proliferation
of
normal
cells
(
Vero
cells
)
was
observed
.
Further
study
revealed
that
chrysin
caused
HepG
2
cell
shrinkage
,
membrane
blebbing
,
and
apoptotic
body
formation
,
all
of
which
were
typical
characteristics
of
apoptosis
programmed
cell
death
.
Flow
cytometric
analysis
demonstrated
that
chrysin
increased
the
sub
G
0
/
G
1
population
,
which
indicated
the
increased
cell
apoptosis
,
thus
preventing
cells
from
entering
the
S
phase
as
the
population
in
G
2
/
M
or
S
phase
declined
;
whereas
in
G
0
/
G
1
phase
,
it
increased
.
In
addition
,
immunoblot
results
showed
that
chrysin
significantly
increased
the
expression
levels
of
caspase-
3
and
Bax
proteins
,
and
it
decreased
the
expression
level
of
B-
cell
lymphoma
/
leukemia
-
2
(
Bcl-
2
)
protein
.
These
findings
indicate
that
chrysin
is
the
major
flavonoid
present
in
DDS
,
and
it
induces
HepG
2
cell
death
via
apoptosis
,
probably
through
the
participation
of
caspase-
3
,
Bax
,
and
Bcl-
2
proteins
.
Diseases
Validation
Diseases presenting
"further study"
symptom
achondroplasia
congenital toxoplasmosis
esophageal adenocarcinoma
esophageal carcinoma
holt-oram syndrome
homocystinuria without methylmalonic aciduria
oculocutaneous albinism
phenylketonuria
pleomorphic liposarcoma
triple a syndrome
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