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BTG1 underexpression is an independent prognostic marker in esophageal squamous cell carcinoma.
[esophageal carcinoma]
To
determine
the
expression
and
function
of
B
cell
translocation
gene
1
(
BTG
1
)
in
esophageal
carcinoma
,
esophageal
samples
were
taken
from
cancer
lesions
(
n
=
74
)
and
adjacent
normal
tissue
(
n
=
34
)
in
esophageal
cancer
patients
immediately
after
endoscopic
biopsy
.
BTG
1
expression
was
determined
by
immunohistochemistry
and
Western
blotting
.
The
effect
of
BTG
1
overexpression
was
examined
in
vitro
utilizing
a
human
esophageal
cancer
cell
line
ECA-
109
stably
transfected
with
a
recombinant
lentivirus
(
LeBTG
1
cells
)
and
compared
to
empty
vector-transfected
controls
(
LeEmpty
)
.
BTG
1
overexpression
was
verified
by
real-time
reverse
transcriptase
polymerase
chain
reaction
(
RT-PCR
)
and
Western
blot
.
The
expression
of
proteins
involved
in
cell
cycle
regulation
(
cyclin
D
1
)
and
apoptosis
(
Bcl-
2
)
and
cell
migration
(
MMP-
9
)
in
LeBTG
1
cells
was
analyzed
by
Western
blot
.
The
effect
of
BTG
1
overexpression
on
cell
viability
and
proliferation
was
assessed
by
an
MTT
assay
in
LeBTG
1
and
LeEmpty
cells
.
Flow
cytometric
analyses
were
used
to
evaluate
the
effect
of
BTG
1
expression
on
cell
cycle
distribution
and
apoptosis
.
The
migration
and
invasion
potential
of
LeBTG
1
cells
was
examined
by
plating
cells
in
Matrigel-coated
chambers
.
The
level
of
BTG
1
protein
expression
was
found
to
be
significantly
lower
in
esophageal
cancer
tissue
than
normal
tissues
(
P
<
0
.
05
)
.
Decreased
expression
of
BTG
1
was
significantly
correlated
with
lymph
node
metastasis
,
clinical
stage
,
and
histological
grade
of
patients
with
esophageal
cancer
(
P
<
0
.
05
)
.
Meanwhile
,
loss
of
BTG
1
expression
correlated
significantly
with
poor
overall
survival
time
by
Kaplan-
Meier
analysis
(
P
<
0
.
05
)
.
The
result
of
biological
function
shown
that
Eca-
109
cell-transfected
BTG
1
had
a
lower
survival
fraction
,
higher
percentage
of
the
G
0
/
G
1
phases
,
higher
cell
apoptosis
,
significant
decrease
in
migration
and
invasion
,
and
lower
cylin
D
1
,
Bcl-
2
,
and
MMP-
9
protein
expression
compared
with
Eca-
109
cell-untransfected
BTG
1
(
P
<
0
.
05
)
.
Reduced
BTG
1
expression
is
associated
with
increased
disease
severity
,
suggesting
it
is
a
negative
regulator
of
esophageal
cancer
and
can
serve
as
a
prognostic
indicator
.
Diseases
Validation
Diseases presenting
"cancer"
symptom
achondroplasia
acute rheumatic fever
adrenal incidentaloma
alpha-thalassemia
benign recurrent intrahepatic cholestasis
cadasil
canavan disease
carcinoma of the gallbladder
cholangiocarcinoma
coats disease
congenital adrenal hyperplasia
congenital diaphragmatic hernia
cowden syndrome
cushing syndrome
cutaneous mastocytosis
dedifferentiated liposarcoma
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
erdheim-chester disease
erythropoietic protoporphyria
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
familial hypocalciuric hypercalcemia
familial mediterranean fever
gm1 gangliosidosis
heparin-induced thrombocytopenia
hereditary cerebral hemorrhage with amyloidosis
hirschsprung disease
hodgkin lymphoma, classical
inclusion body myositis
junctional epidermolysis bullosa
kabuki syndrome
kallmann syndrome
kindler syndrome
lamellar ichthyosis
liposarcoma
locked-in syndrome
lymphangioleiomyomatosis
monosomy 21
neuralgic amyotrophy
oculocutaneous albinism
oligodontia
oral submucous fibrosis
papillon-lefèvre syndrome
pendred syndrome
pleomorphic liposarcoma
primary effusion lymphoma
proteus syndrome
pyomyositis
pyruvate dehydrogenase deficiency
severe combined immunodeficiency
sneddon syndrome
systemic capillary leak syndrome
triple a syndrome
von hippel-lindau disease
waldenström macroglobulinemia
well-differentiated liposarcoma
werner syndrome
wiskott-aldrich syndrome
wolf-hirschhorn syndrome
x-linked adrenoleukodystrophy
This symptom has already been validated