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Effects of PLCE1 gene silencing by RNA interference on cell cycling and apoptosis in esophageal carcinoma cells.
[esophageal carcinoma]
Esophageal
squamous
cell
carcinoma
(
ESCC
)
is
one
of
the
most
malignancies
with
a
poor
prognosis
.
The
phospholipase
C
?
gene
(
PLCE
1
)
encodes
a
novel
ras-related
protein
effector
mediating
the
effects
of
R-Ras
on
the
actin
cytoskeleton
and
membrane
protrusion
.
However
,
molecular
mechanisms
pertinent
to
ESCC
are
unclear
.
We
therefore
designed
PLCE
1
-
special
small
interfering
RNA
and
transfected
to
esophageal
squamous
cell
(
EC
)
9706
cells
to
investigate
the
effects
of
PLCE
1
gene
silencing
on
the
cell
cycle
and
apoptosis
of
ESCC
and
indicate
its
important
role
in
the
development
of
ESCC
.
Esophageal
cancer
tissue
specimens
and
normal
esophageal
mucosa
were
obtained
and
assayed
by
immunohistochemical
staining
to
confirm
overexpression
of
PLCE
1
in
neoplasias
.
Fluorescence
microscopy
was
used
to
examine
transfection
efficiency
,
while
the
result
of
PLCE
1
silencing
was
examined
by
reverse
transcription
(
RT-PCR
)
.
Flow
cytometry
and
annexin
V
apoptosis
assays
were
used
to
assess
the
cell
cycle
and
apoptosis
,
respectively
.
Expression
of
cyclin
D
1
and
caspase-
3
was
detected
by
Western
-blotting
.
The
level
of
PLCE
1
protein
in
esophageal
cancer
tissue
was
significantly
higher
than
that
in
normal
tissue
.
After
transfection
,
the
expression
of
PLCE
1
mRNA
in
EC
9706
was
significantly
reduced
,
compared
with
the
control
group
.
Furthermore
,
flow
cytometry
results
suggested
that
the
PLCE
1
gene
silencing
arrested
the
cell
cycle
in
the
G
0
/
G
1
phase
;
apoptosis
was
significantly
higher
than
in
the
negative
control
group
and
mock
group
.
PLCE
1
gene
silencing
by
RNAi
resulted
in
decreased
expression
of
cyclin
D
1
and
increased
expression
of
caspase-
3
.
Our
study
suggests
that
PLCE
1
may
be
an
oncogene
and
play
an
important
role
in
esophageal
carcinogenesis
through
regulating
proteins
which
control
cell
cycling
and
apoptosis
.
Diseases
Validation
Diseases presenting
"squamous cell carcinoma"
symptom
carcinoma of the gallbladder
child syndrome
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
junctional epidermolysis bullosa
kallmann syndrome
kindler syndrome
liposarcoma
monosomy 21
oculocutaneous albinism
oral submucous fibrosis
papillon-lefèvre syndrome
This symptom has already been validated