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TPX2 siRNA regulates growth and invasion of esophageal cancer cells.
[esophageal carcinoma]
Observe
how
specific
small
RNA
interference
(
siRNA
)
aimed
at
TPX
2
gene
suppresses
TPX
2
gene
expression
in
esophageal
cancer
EC
9706
cells
and
the
effect
on
esophageal
cancer
cell
growth
and
invasion
ability
.
Transfect
TPX
2
siRNA
into
EC
9706
cells
via
lipofectamin
2000
.
The
experiments
were
divided
into
three
groups
,
a
negative
control
,
a
blank
control
and
an
siRNA
interference
group
(
24
h
,
48
h
,
72
h
,
96
h
)
.
We
examined
RNA
and
protein
level
alteration
of
the
TPX
2
gene
after
TPX
2
siRNA
transfection
by
RT-PCR
and
Western
blot
analysis
.
Detection
of
how
TPX
2
siRNA
influences
EC
9706
cell
proliferation
was
done
by
MTT
,
cell
apoptosis
monitored
through
Tunel
assay
,
in
vitro
invasion
ability
via
Boyden
chamber
and
cell
cycle
change
by
flow
cytometry
.
After
effective
siRNA
transfection
,
TPX
2
mRNA
and
protein
expression
level
in
siRNA
interference
group
were
(
0
.
31
±
0
.
08
,
0
.
39
±
0
.
12
)
,
72
h
after
transfection
,
significantly
lower
than
blank
control
group
(
1
.
00
±
0
.
01
)
and
negative
control
group
(
0
.
98
±
0
.
11
)
,
(
F
=
71
.
182
,
t
1
=
8
.
17
,
t
2
=
7
.
90
,
P
<
0
.
05
)
;
MTT
results
demonstrated
that
cell
growth
and
proliferation
were
inhibited
and
the
inhibition
rate
was
up
to
35
.
4
%
(
P
<
0
.
05
)
compared
with
the
control
group
.
TUNEL
results
indicated
that
cell
apoptosis
index
in
siRNA
interference
group
was
18
.
28
±
0
.
35
,
higher
than
that
in
blank
control
group
(
4
.
07
±
0
.
26
)
and
negative
control
group
(
4
.
13
±
0
.
22
)
,
(
F
=
244
.
5
,
t
1
=
60
.
61
,
t
2
=
53
.
32
,
P
<
0
.
01
)
.
Boyden
chamber
results
showed
that
the
transmembrane
cell
number
was
45
.
30
±
8
.
08
in
siRNA
interference
group
,
less
than
blank
control
group
(
121
.
90
±
7
.
83
)
,
(
F
=
122
.
46
,
t
1
=
11
.
81
,
t
2
=
10
.
47
,
P
<
0
.
01
)
;
besides
,
in
siRNA
interference
group
cell
invasion
inhibition
rate
was
71
.
42
±
9
.
12
,
higher
than
negative
control
group
(
5
.
65
±
3
.
55
)
,
(
t
=
14
.
256
,
P
<
0
.
01
)
.
Flow
cytometry
results
illustrated
that
more
EC
9706
cells
went
into
apoptosis
and
cell
cycle
arrested
in
S
phase
.
Similar
results
were
obtained
by
in
vivo
transplantation
,
as
TPX
2
siRNA
transfection
significantly
reduced
tumor
growth
of
the
xenograft
in
nude
mice
.
siRNA
could
effectively
inhibit
the
invasion
and
metastasis
of
EC
9706
cells
,
promote
the
apoptosis
of
tumor
cells
and
may
become
a
new
approach
for
treatment
of
esophageal
carcinoma
.
Diseases
Validation
Diseases presenting
"tumor cells"
symptom
alpha-thalassemia
carcinoma of the gallbladder
cholangiocarcinoma
cushing syndrome
dedifferentiated liposarcoma
dentin dysplasia
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
hodgkin lymphoma, classical
junctional epidermolysis bullosa
kindler syndrome
liposarcoma
lymphangioleiomyomatosis
pleomorphic liposarcoma
primary effusion lymphoma
severe combined immunodeficiency
triple a syndrome
von hippel-lindau disease
waldenström macroglobulinemia
well-differentiated liposarcoma
werner syndrome
wiskott-aldrich syndrome
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