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TPX2 siRNA regulates growth and invasion of esophageal cancer cells.
[esophageal carcinoma]
Observe
how
specific
small
RNA
interference
(
siRNA
)
aimed
at
TPX
2
gene
suppresses
TPX
2
gene
expression
in
esophageal
cancer
EC
9706
cells
and
the
effect
on
esophageal
cancer
cell
growth
and
invasion
ability
.
Transfect
TPX
2
siRNA
into
EC
9706
cells
via
lipofectamin
2000
.
The
experiments
were
divided
into
three
groups
,
a
negative
control
,
a
blank
control
and
an
siRNA
interference
group
(
24
h
,
48
h
,
72
h
,
96
h
)
.
We
examined
RNA
and
protein
level
alteration
of
the
TPX
2
gene
after
TPX
2
siRNA
transfection
by
RT-PCR
and
Western
blot
analysis
.
Detection
of
how
TPX
2
siRNA
influences
EC
9706
cell
proliferation
was
done
by
MTT
,
cell
apoptosis
monitored
through
Tunel
assay
,
in
vitro
invasion
ability
via
Boyden
chamber
and
cell
cycle
change
by
flow
cytometry
.
After
effective
siRNA
transfection
,
TPX
2
mRNA
and
protein
expression
level
in
siRNA
interference
group
were
(
0
.
31
±
0
.
08
,
0
.
39
±
0
.
12
)
,
72
h
after
transfection
,
significantly
lower
than
blank
control
group
(
1
.
00
±
0
.
01
)
and
negative
control
group
(
0
.
98
±
0
.
11
)
,
(
F
=
71
.
182
,
t
1
=
8
.
17
,
t
2
=
7
.
90
,
P
<
0
.
05
)
;
MTT
results
demonstrated
that
cell
growth
and
proliferation
were
inhibited
and
the
inhibition
rate
was
up
to
35
.
4
%
(
P
<
0
.
05
)
compared
with
the
control
group
.
TUNEL
results
indicated
that
cell
apoptosis
index
in
siRNA
interference
group
was
18
.
28
±
0
.
35
,
higher
than
that
in
blank
control
group
(
4
.
07
±
0
.
26
)
and
negative
control
group
(
4
.
13
±
0
.
22
)
,
(
F
=
244
.
5
,
t
1
=
60
.
61
,
t
2
=
53
.
32
,
P
<
0
.
01
)
.
Boyden
chamber
results
showed
that
the
transmembrane
cell
number
was
45
.
30
±
8
.
08
in
siRNA
interference
group
,
less
than
blank
control
group
(
121
.
90
±
7
.
83
)
,
(
F
=
122
.
46
,
t
1
=
11
.
81
,
t
2
=
10
.
47
,
P
<
0
.
01
)
;
besides
,
in
siRNA
interference
group
cell
invasion
inhibition
rate
was
71
.
42
±
9
.
12
,
higher
than
negative
control
group
(
5
.
65
±
3
.
55
)
,
(
t
=
14
.
256
,
P
<
0
.
01
)
.
Flow
cytometry
results
illustrated
that
more
EC
9706
cells
went
into
apoptosis
and
cell
cycle
arrested
in
S
phase
.
Similar
results
were
obtained
by
in
vivo
transplantation
,
as
TPX
2
siRNA
transfection
significantly
reduced
tumor
growth
of
the
xenograft
in
nude
mice
.
siRNA
could
effectively
inhibit
the
invasion
and
metastasis
of
EC
9706
cells
,
promote
the
apoptosis
of
tumor
cells
and
may
become
a
new
approach
for
treatment
of
esophageal
carcinoma
.
Diseases
Validation
Diseases presenting
"tumor growth of the xenograft in nude mice"
symptom
esophageal carcinoma
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