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Pimecrolimus induces apoptosis of mast cells in a murine model of cutaneous mastocytosis.
[cutaneous mastocytosis]
Cutaneous
mastocytosis
(
CM
)
is
a
common
type
of
mastocytosis
.
Current
treatment
of
CM
is
generally
symptomatic
.
Pimecrolimus
has
been
demonstrated
as
an
effective
anti-
inflammatory
drug
for
the
treatment
of
inflammatory
skin
diseases
,
but
whether
it
treats
CM
remains
unknown
.
The
murine
model
of
CM
was
induced
by
subcutaneous
injection
of
100
μg
/
kg
recombinant
murine
stem
cell
factor
(
rmSCF
)
for
a
total
of
17
days
in
Balb
/
c
mice
.
Beginning
on
the
8
th
day
,
treatment
with
pimecrolimus
1
%
cream
or
vehicle
was
performed
topically
and
daily
for
10
days
.
The
clinical
signs
of
CM
were
scored
,
and
pathological
analysis
was
performed
with
toluidine
blue
staining
and
hematoxylin
and
eosin
staining
.
The
in
situ
apoptotic
mast
cells
(
MCs
)
were
studied
by
terminal
deoxynucleotidyl
transferase-mediated
dUTP
nick
-end
labeling
assay
.
The
cutaneous
histamine
level
was
measured
by
ELISA
.
In
the
rmSCF-treated
mice
,
the
clinical
signs
of
CM
,
including
erythema
,
wheal
after
rubbing
lesion
skins
,
and
increased
thickness
of
skin
,
were
obvious
compared
to
control
mice
,
and
were
reduced
after
pimecrolimus
treatment
.
The
numbers
of
cutaneous
MCs
and
neutrophils
were
significantly
greater
in
mice
with
CM
than
in
control
mice
,
and
pimecrolimus
treatment
decreased
the
numbers
of
MCs
but
not
neutrophils
.
Extensive
apoptosis
of
cutaneous
MCs
was
observed
in
pimecrolimus-treated
mice
.
The
cutaneous
histamine
level
was
elevated
in
the
mice
with
CM
compared
with
healthy
controls
,
and
was
lowered
after
treatment
with
pimecrolimus
.
Pimecrolimus
effectively
treats
CM
by
reducing
the
density
of
cutaneous
MCs
and
the
subsequent
histamine
production
through
inducing
MCs
apoptosis
.
Diseases
Validation
Diseases presenting
"cutaneous histamine level"
symptom
cutaneous mastocytosis
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