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PD-1 regulates the growth of human mastocytosis cells.
[cutaneous mastocytosis]
Programmed
death-
1
(
PD-
1
)
is
a
marker
for
human
neoplastic
T
cells
.
Here
,
we
evaluated
whether
or
not
PD-
1
was
also
a
marker
for
human
mastocytosis
,
and
explored
the
role
of
PD-
1
in
human
mastocytosis
cells
.
Immunohistochemical
analysis
was
used
to
evaluate
the
expression
of
PD-
1
in
clinical
samples
of
human
cutaneous
mastocytosis
.
The
expression
of
PD-
1
in
human
mastocytosis
cell
lines
was
checked
by
RT-PCR
,
western
blotting
and
flow
cytometry
.
We
stimulated
human
mastocytosis
cell
lines
(
LAD
2
and
HMC
1
.
2
)
with
recombinant
ligand
for
PD-
1
,
PD-L
1
(
rPD-L
1
)
,
and
tested
the
proliferative
activity
and
the
status
of
signal
molecules
by
Cell
Counting
Kit-
8
and
ELISA
,
respectively
.
Ten
of
30
human
cutaneous
mastocytosis
cases
(
33
.
3
%
)
expressed
PD-
1
protein
.
We
also
found
that
a
human
mastocytosis
line
LAD
2
cells
expressed
PD-
1
protein
on
their
surfaces
.
The
administration
with
rPD-L
1
suppressed
the
stem
cell
factor
-dependent
growth
of
the
LAD
2
cells
.
And
,
rPD-L
1
activated
SHP-
1
and
SHP-
2
simultaneously
,
and
decreased
the
phosphorylation
of
AKT
,
in
LAD
2
cells
.
In
contrast
,
we
could
not
detect
the
expression
of
PD-
1
,
and
the
significant
effect
of
rPD-L
1
on
the
mutated
KIT
-driven
growth
of
HMC
1
.
2
cells
.
PD-
1
could
be
a
marker
for
human
cutaneous
mastocytosis
and
regulate
the
growth
of
human
PD-
1
-
positive
mastocytosis
cells
.
Diseases
Validation
Diseases presenting
"human neoplastic t cells"
symptom
cutaneous mastocytosis
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