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Therapeutic potential of mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model.
[congenital diaphragmatic hernia]
The
aim
of
this
study
was
to
evaluate
the
efficacy
of
mesenchymal
stem
cells
(
MSCs
)
in
a
nitrofen-induced
congenital
diaphragmatic
hernia
(
CDH
)
rat
model
.
Pregnant
rats
were
exposed
to
nitrofen
on
embryonic
day
9
.
5
(
E
9
.
5
)
.
MSCs
were
isolated
from
the
enhanced
green
fluorescent
protein
(
eGFP
)
transgenic
rat
lungs
.
The
MSCs
were
transplanted
into
the
nitrofen-induced
E
12
.
5
rats
via
the
uterine
vein
,
and
the
E
21
lung
explants
were
harvested
.
The
study
animals
were
divided
into
three
:
the
control
group
,
the
nitrofen-induced
left
CDH
(
CDH
group
)
,
and
the
MSC
-treated
nitrofen-induced
left
CDH
(
MSC
-treated
CDH
group
)
.
The
specimens
were
morphologically
analyzed
using
HE
and
immunohistochemical
staining
with
proliferating
cell
nuclear
antigen
(
PCNA
)
,
surfactant
protein-
C
(
SP-C
)
,
and
α-smooth
muscle
actin
.
The
alveolar
and
medial
walls
of
the
pulmonary
arteries
were
significantly
thinner
in
the
MSC
-treated
CDH
group
than
in
the
CDH
group
.
The
alveolar
air
space
areas
were
larger
,
while
PCNA
and
the
SP-C
positive
cells
were
significantly
higher
in
the
MSC
-treated
CDH
group
,
than
in
the
CDH
group
.
MSC
engraftment
was
identified
on
immunohistochemical
staining
of
the
GFP
in
the
MSC
-treated
CDH
group
.
MSC
transplantation
potentially
promotes
alveolar
and
pulmonary
artery
development
,
thereby
reducing
the
severity
of
pulmonary
hypoplasia
.
Diseases
Validation
Diseases presenting
"positive cells"
symptom
canavan disease
carcinoma of the gallbladder
coats disease
congenital diaphragmatic hernia
dedifferentiated liposarcoma
epidermolysis bullosa simplex
erythropoietic protoporphyria
esophageal adenocarcinoma
hodgkin lymphoma, classical
severe combined immunodeficiency
werner syndrome
x-linked adrenoleukodystrophy
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