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Therapeutic potential of mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model.
[congenital diaphragmatic hernia]
The
aim
of
this
study
was
to
evaluate
the
efficacy
of
mesenchymal
stem
cells
(
MSCs
)
in
a
nitrofen-induced
congenital
diaphragmatic
hernia
(
CDH
)
rat
model
.
Pregnant
rats
were
exposed
to
nitrofen
on
embryonic
day
9
.
5
(
E
9
.
5
)
.
MSCs
were
isolated
from
the
enhanced
green
fluorescent
protein
(
eGFP
)
transgenic
rat
lungs
.
The
MSCs
were
transplanted
into
the
nitrofen-induced
E
12
.
5
rats
via
the
uterine
vein
,
and
the
E
21
lung
explants
were
harvested
.
The
study
animals
were
divided
into
three
:
the
control
group
,
the
nitrofen-induced
left
CDH
(
CDH
group
)
,
and
the
MSC
-treated
nitrofen-induced
left
CDH
(
MSC
-treated
CDH
group
)
.
The
specimens
were
morphologically
analyzed
using
HE
and
immunohistochemical
staining
with
proliferating
cell
nuclear
antigen
(
PCNA
)
,
surfactant
protein-
C
(
SP-C
)
,
and
α-smooth
muscle
actin
.
The
alveolar
and
medial
walls
of
the
pulmonary
arteries
were
significantly
thinner
in
the
MSC
-treated
CDH
group
than
in
the
CDH
group
.
The
alveolar
air
space
areas
were
larger
,
while
PCNA
and
the
SP-C
positive
cells
were
significantly
higher
in
the
MSC
-treated
CDH
group
,
than
in
the
CDH
group
.
MSC
engraftment
was
identified
on
immunohistochemical
staining
of
the
GFP
in
the
MSC
-treated
CDH
group
.
MSC
transplantation
potentially
promotes
alveolar
and
pulmonary
artery
development
,
thereby
reducing
the
severity
of
pulmonary
hypoplasia
.
