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Determination of six pterins in urine by LC-MS/MS.
[classical phenylketonuria]
The
present
work
describes
an
analytical
method
for
urinary
pterins
by
LC
-
MS
/
MS
,
with
emphasis
on
the
separation
of
6
-
and
7
-
positional
isomers
of
bio-
and
neopterins
.
Urine
sample
preparation
consisted
of
oxidation
by
MnO
(
2
)
,
filtration
and
direct
dilution
in
the
mobile
phase
.
The
method
was
validated
in
urine
spiked
at
five
concentration
levels
with
true
triplicates
of
each
level
.
Separation
of
the
pterins
,
including
the
positional
isomers
,
was
achieved
by
employing
a
LUNA
amino
column
.
Six
pterins
were
quantified
(
pterin
,
isoxanthopterin
,
6
-
biopterin
,
7
-
biopterin
,
6
-
neopterin
,
7
-
neopterin
)
and
a
linear
behavior
was
observed
;
LOD
varied
from
7
to
360
pg
/
ml
and
correlation
coefficients
above
0
.
98
were
obtained
for
all
pterins
.
In
addition
,
pterin
levels
were
evaluated
in
41
urine
samples
of
healthy
subjects
,
in
ten
urine
samples
of
patients
with
classical
phenylketonuria
(
PKU
)
and
in
one
with
atypical
PKU
.
T
he
proposed
method
allowed
to
identify
,
separate
and
quantify
six
pterins
in
urine
,
using
a
simple
and
rapid
sample
preparation
.
The
atypical
PKU
was
unequivocally
differentiated
from
the
classical
form
,
demonstrating
that
this
method
could
be
very
useful
for
characterization
and
follow-up
of
diseases
.
Diseases
Validation
Diseases presenting
"urinary pterins"
symptom
classical phenylketonuria
phenylketonuria
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