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Defensive mechanism in cholangiocarcinoma cells against oxidative stress induced by chlorin e6-based photodynamic therapy.
[cholangiocarcinoma]
In
this
study
,
the
effect
of
chlorin
e
6
-
based
photodynamic
therapy
(
Ce
6
-
PDT
)
was
investigated
in
human
intrahepatic
(
HuCC-
T
1
)
and
extrahepatic
(
SNU
1196
)
cholangiocarcinoma
(
CCA
)
cells
.
The
amount
of
intracellular
Ce
6
increased
with
increasing
Ce
6
concentration
administered
,
or
with
incubation
time
,
in
both
cell
lines
.
The
ability
to
take
up
Ce
6
and
generate
reactive
oxygen
species
after
irradiation
at
1
.
0
J
/
cm
(
2
)
did
not
significantly
differ
between
the
two
CCA
cell
types
.
However
,
after
irradiation
,
marked
differences
were
observed
for
photodamage
and
apoptotic
/
necrotic
signals
.
HuCC-
T
1
cells
are
more
sensitive
to
Ce
6
-
PDT
than
SNU
1196
cells
.
Total
glutathione
(
GSH
)
levels
,
glutathione
peroxidase
and
glutathione
reductase
activities
in
SNU
1196
cells
were
significantly
higher
than
in
HuCC-
T
1
cells
.
With
inhibition
of
enzyme
activity
or
addition
of
GSH
,
the
phototoxic
effect
could
be
controlled
in
CCA
cells
.
The
intracellular
level
of
GSH
is
the
most
important
determining
factor
in
the
curative
action
of
Ce
6
-
PDT
against
tumor
cells
.
