Rare Diseases Symptoms Automatic Extraction
Home
A random Abstract
Our Project
Our Team
Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts.
[cholangiocarcinoma]
Decitabine
(
DAC
)
,
an
inhibitor
of
DNA
methyltransferase
,
demonstrates
antitumor
activities
in
various
types
of
cancer
.
However
,
its
therapeutic
potential
for
cholangiocarcinoma
(
CCA
)
,
one
of
the
most
aggressive
gastrointestinal
malignancies
,
remains
to
be
explored
.
The
present
study
investigated
the
antiproliferative
effects
of
DAC
on
CCA
cells
in
vitro
and
in
vivo
.
Human
CCA
cell
lines
,
TFK-
1
and
QBC
939
,
were
used
as
models
to
investigate
DAC
on
the
cell
growth
and
proliferation
of
CCA
.
Cell
proliferation
was
evaluated
by
Cell
Counting
Kit-
8
assay
combined
with
clonogenic
survival
assay
.
Flow
cytometry
,
Hoechst
33342
/
propidium
iodide
staining
and
green
fluorescent
protein-tagged
MAP-
LC
3
detection
were
applied
to
determine
cell
cycle
progression
,
apoptosis
and
autophagy
.
Nude
mice
with
TFK-
1
xenografts
were
evaluated
for
tumor
growth
following
DAC
treatment
.
DAC
was
observed
to
significantly
suppress
the
proliferation
of
cultured
TFK-
1
and
QBC
939
cells
,
accompanied
with
enhanced
apoptosis
,
autophagy
and
cell
cycle
arrest
at
G
2
/
M
phase
.
In
TFK-
1
mouse
xenografts
,
DAC
retarded
the
tumor
growth
and
increased
the
survival
of
CCA
tumor
-bearing
mice
.
Diseases
Validation
Diseases presenting
"increased the survival of cca tumor-bearing mice"
symptom
cholangiocarcinoma
You can validate or delete this automatically detected symptom
Validate the Symptom
Delete the Symptom