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Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts.
[cholangiocarcinoma]
Decitabine
(
DAC
)
,
an
inhibitor
of
DNA
methyltransferase
,
demonstrates
antitumor
activities
in
various
types
of
cancer
.
However
,
its
therapeutic
potential
for
cholangiocarcinoma
(
CCA
)
,
one
of
the
most
aggressive
gastrointestinal
malignancies
,
remains
to
be
explored
.
The
present
study
investigated
the
antiproliferative
effects
of
DAC
on
CCA
cells
in
vitro
and
in
vivo
.
Human
CCA
cell
lines
,
TFK-
1
and
QBC
939
,
were
used
as
models
to
investigate
DAC
on
the
cell
growth
and
proliferation
of
CCA
.
Cell
proliferation
was
evaluated
by
Cell
Counting
Kit-
8
assay
combined
with
clonogenic
survival
assay
.
Flow
cytometry
,
Hoechst
33342
/
propidium
iodide
staining
and
green
fluorescent
protein-tagged
MAP-
LC
3
detection
were
applied
to
determine
cell
cycle
progression
,
apoptosis
and
autophagy
.
Nude
mice
with
TFK-
1
xenografts
were
evaluated
for
tumor
growth
following
DAC
treatment
.
DAC
was
observed
to
significantly
suppress
the
proliferation
of
cultured
TFK-
1
and
QBC
939
cells
,
accompanied
with
enhanced
apoptosis
,
autophagy
and
cell
cycle
arrest
at
G
2
/
M
phase
.
In
TFK-
1
mouse
xenografts
,
DAC
retarded
the
tumor
growth
and
increased
the
survival
of
CCA
tumor
-bearing
mice
.
Diseases
Validation
Diseases presenting
"tumor growth"
symptom
adrenal incidentaloma
carcinoma of the gallbladder
cholangiocarcinoma
cowden syndrome
cystinuria
esophageal adenocarcinoma
esophageal carcinoma
esophageal squamous cell carcinoma
hodgkin lymphoma, classical
liposarcoma
lymphangioleiomyomatosis
primary effusion lymphoma
severe combined immunodeficiency
von hippel-lindau disease
waldenström macroglobulinemia
wiskott-aldrich syndrome
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