Rare Diseases Symptoms Automatic Extraction
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Microvascular damage is involved in the pathogenesis of heroin induced spongiform leukoencephalopathy.
[canavan disease]
To
investigate
whether
microvascular
damage
is
involved
in
the
pathogenesis
of
heroin
induced
spongiform
leukoencephalopathy
(
HSLE
)
.
The
brain
tissues
were
collected
from
4
HSLE
patients
and
5
controls
and
then
fixed
in
4
%
paraformaldehyde
.
The
frontal
lobe
,
corpus
callosum
and
cerebellum
were
separated
.
The
expressions
of
myelin
base
protein
(
MBP
)
and
CD
34
were
detected
by
immunohistochemistry
.
TUNEL
staining
was
applied
to
detect
cell
apoptosis
.
The
correlation
between
microvascular
changes
and
pathological
vacuoles
was
evaluated
.
No
obvious
abnormalities
were
found
in
the
brain
of
controls
.
Immunohistochemistry
for
MBP
showed
the
collapse
and
fracture
of
myelin
sheath
and
vacuole
formation
in
the
subcortical
white
matter
,
corpus
callosum
,
and
cerebellar
white
matter
of
HSLE
patients
.
TUNEL
staining
showed
the
number
of
apoptotic
cells
in
the
cerebellar
white
matter
and
corpus
callosum
of
HSLE
patients
was
significantly
higher
than
that
in
controls
(
F
=
389
.
451
,
P
<
0
.
001
)
.
Masson
's
trichrome
staining
revealed
vacuolar
degeneration
in
the
cerebral
white
matter
of
HSLE
patients
,
and
the
vacuoles
were
distributed
around
the
microvessels
.
Immunohistochemistry
revealed
CD
34
positive
cells
were
seldom
found
besides
the
vessels
in
the
cerebellar
white
matter
and
corpus
callosum
of
HSLE
patients
,
but
a
variety
of
CD
34
positive
cells
was
found
in
the
vascular
wall
of
controls
(
F
=
838
.
500
,
P
<
0
.
001
)
.
Apoptosis
of
oligodendrocytes
may
be
related
to
the
HSLE
.
Cerebral
vascular
injury
and
microcirculation
dysfunction
are
involved
in
the
pathogenesis
of
HSLE
.
The
interrelation
between
apoptosis
of
oligodendrocytes
and
the
microvascular
damage
are
required
to
be
studied
in
future
investigations
.
