Ganoderiol A-enriched extract suppresses migration and adhesion of MDA-MB-231 cells by inhibiting FAK-SRC-paxillin cascade pathway.

[wiskott-aldrich syndrome]

Cell adhesion , migration and invasion are critical steps for carcinogenesis and cancer metastasis . Ganoderma lucidum , also called Lingzhi in China , is a traditional Chinese medicine , which exhibits anti-proliferation , anti-inflammation and anti-metastasis properties . Herein , GAEE , G . lucidum extract mainly contains ganoderiol A (GA ), dihydrogenated GA and GA isomer , was shown to inhibit the abilities of adhesion and migration , while have a slight influence on that of invasion in highly metastatic breast cancer MDA-MB -231 cells at non-toxic doses . Further investigation revealed that GAEE decreased the active forms of focal adhesion kinase (FAK ) and disrupted the interaction between FAK and SRC , which lead to deactivating of paxillin . Moreover , GAEE treatment downregulated the expressions of RhoA , Rac 1 , and Cdc 42 , and decreased the interaction between neural Wiskott-Aldrich Syndrome protein (N-WASP ) and Cdc 42 , which impair cell migration and actin assembly . To our knowledge , this is the first report to show that G .lucidum triterpenoids could suppress cell migration and adhesion through FAK-SRC -paxillin signaling pathway . Our study also suggests that GAEE may be a potential agent for treatment of breast cancer .

Diseases
Validation

Diseases presenting "breast cancer" symptom

acute rheumatic fever

aromatase deficiency

carcinoma of the gallbladder

child syndrome

cowden syndrome

cutaneous mastocytosis

dedifferentiated liposarcoma

esophageal squamous cell carcinoma

junctional epidermolysis bullosa

kindler syndrome

liposarcoma

lymphangioleiomyomatosis

oral submucous fibrosis

proteus syndrome

severe combined immunodeficiency

systemic capillary leak syndrome

von hippel-lindau disease

waldenström macroglobulinemia

werner syndrome

wiskott-aldrich syndrome

This symptom has already been validated