Ganoderiol A-enriched extract suppresses migration and adhesion of MDA-MB-231 cells by inhibiting FAK-SRC-paxillin cascade pathway.

[wiskott-aldrich syndrome]

Cell adhesion , migration and invasion are critical steps for carcinogenesis and cancer metastasis . Ganoderma lucidum , also called Lingzhi in China , is a traditional Chinese medicine , which exhibits anti-proliferation , anti-inflammation and anti-metastasis properties . Herein , GAEE , G . lucidum extract mainly contains ganoderiol A (GA ), dihydrogenated GA and GA isomer , was shown to inhibit the abilities of adhesion and migration , while have a slight influence on that of invasion in highly metastatic breast cancer MDA-MB -231 cells at non-toxic doses . Further investigation revealed that GAEE decreased the active forms of focal adhesion kinase (FAK ) and disrupted the interaction between FAK and SRC , which lead to deactivating of paxillin . Moreover , GAEE treatment downregulated the expressions of RhoA , Rac 1 , and Cdc 42 , and decreased the interaction between neural Wiskott-Aldrich Syndrome protein (N-WASP ) and Cdc 42 , which impair cell migration and actin assembly . To our knowledge , this is the first report to show that G .lucidum triterpenoids could suppress cell migration and adhesion through FAK-SRC -paxillin signaling pathway . Our study also suggests that GAEE may be a potential agent for treatment of breast cancer .

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Diseases presenting "traditional chinese medicine" symptom

wiskott-aldrich syndrome

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