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Mutagenetic and electron microscopy analysis of actin filament severing by Cordon-Bleu, a WH2 domain protein.
[wiskott-aldrich syndrome]
Cordon-
Bleu
(
Cobl
)
is
a
regulator
of
actin
dynamics
in
neural
development
and
ciliogenesis
.
Its
function
is
associated
with
three
adjacent
actin
binding
WASP
Homology
2
(
WH
2
)
domains
.
We
showed
that
these
WH
2
repeats
confer
multifunctional
regulation
of
actin
dynamics
,
which
makes
Cobl
a
«
dynamizer
»
of
actin
assembly
,
inducing
fast
turnover
of
actin
filaments
and
oscillatory
polymerization
regime
via
nucleation
,
severing
,
and
rapid
depolymerization
activities
.
Cobl
is
the
most
efficient
severer
of
actin
filaments
characterized
so
far
.
To
understand
which
primary
sequence
elements
determine
the
filament
severing
activity
of
the
WH
2
repeats
,
here
we
combine
a
mutagenetic
/
domain
swapping
approach
of
the
minimal
fully
active
Cobl-
KAB
construct
,
which
comprises
the
lysine
rich
region
K
preceding
the
two
first
WH
2
domains
A
and
B
.
The
mutated
Cobl
constructs
display
variable
loss
of
the
original
filament
nucleating
activities
of
native
Cobl-
KAB
,
without
any
strict
correlation
with
a
loss
in
actin
binding
,
which
emphasizes
the
functional
importance
of
the
electrostatic
environment
of
WH
2
domains
.
Filament
severing
displayed
the
greatest
stringency
and
was
abolished
in
all
mutated
forms
of
Cobl-
KAB
.
Filament
severing
and
re
-annealing
by
Cobl-
KAB
,
which
is
key
in
its
rapid
remodeling
of
a
population
of
actin
filaments
,
and
most
likely
responsible
for
its
function
in
ciliogenesis
,
was
analyzed
by
electron
microscopy
in
comparison
with
Spire
and
ADF
.
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"primary sequence elements"
symptom
wiskott-aldrich syndrome
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