Rare Diseases Symptoms Automatic Extraction
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A random Abstract
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WAVE regulatory complex activation.
[wiskott-aldrich syndrome]
The
WAVE
regulatory
complex
(
WRC
)
is
critical
to
control
of
actin
polymerization
at
the
eukaryotic
cell
membrane
.
By
reconstituting
WAVE-dependent
actin
assembly
on
silica
microspheres
coated
with
phospholipid
bilayers
in
mammalian
brain
extracts
,
we
discovered
that
membrane
recruitment
and
activation
of
WRC
require
the
cooperative
action
of
two
mammalian
GTPases
,
Arf
and
Rac
.
Here
,
we
describe
detailed
methods
to
generate
phospholipid-coated
microspheres
and
porcine
brain
extract
and
outline
conditions
necessary
to
reconstitute
WRC-dependent
motility
.
In
addition
,
we
describe
how
to
generate
acylated
recombinant
GTPases
,
anchor
them
to
lipid-coated
microspheres
,
and
reconstitute
GTPase
activation
of
WRC
.