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Dissecting principles governing actin assembly using yeast extracts.
[wiskott-aldrich syndrome]
In
this
chapter
,
we
describe
recent
protocols
that
we
have
developed
to
trigger
actin
assembly
and
actin-based
motility
in
yeast
cell
extracts
.
Our
method
allows
for
the
fast
preparation
of
yeast
extracts
that
are
competent
in
dynamic
assembly
of
distinct
actin
filament
structures
of
biologically
appropriate
protein
composition
.
Compared
to
previous
extract-based
systems
using
other
eukaryotic
cell
types
,
yeast
provides
a
unique
advantage
for
combining
reconstituted
assays
with
the
preparation
of
extracts
from
genetically
modified
yeast
strains
.
We
present
a
global
strategy
for
dissecting
the
functions
of
individual
proteins
,
where
the
activities
of
the
proteins
are
analyzed
in
systems
of
variable
complexity
,
ranging
from
simple
mixtures
of
pure
proteins
to
the
full
complexity
of
a
cell
's
cytoplasm
.
Diseases
Validation
Diseases presenting
"global strategy"
symptom
dracunculiasis
wiskott-aldrich syndrome
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