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Strand exchange of telomeric DNA catalyzed by the Werner syndrome protein (WRN) is specifically stimulated by TRF2.
[werner syndrome]
Werner
syndrome
(
WS
)
,
caused
by
loss
of
function
of
the
RecQ
helicase
WRN
,
is
a
hereditary
disease
characterized
by
premature
aging
and
elevated
cancer
incidence
.
WRN
has
DNA
binding
,
exonuclease
,
ATPase
,
helicase
and
strand
annealing
activities
,
suggesting
possible
roles
in
recombination-related
processes
.
Evidence
indicates
that
WRN
deficiency
causes
telomeric
abnormalities
that
likely
underlie
early
onset
of
aging
phenotypes
in
WS
.
Furthermore
,
TRF
2
,
a
protein
essential
for
telomere
protection
,
interacts
with
WRN
and
influences
its
basic
helicase
and
exonuclease
activities
.
However
,
these
studies
provided
little
insight
into
WRN
's
specific
function
at
telomeres
.
Here
,
we
explored
the
possibility
that
WRN
and
TRF
2
cooperate
during
telomeric
recombination
processes
.
Our
results
indicate
that
TRF
2
,
through
its
interactions
with
both
WRN
and
telomeric
DNA
,
stimulates
WRN
-mediated
strand
exchange
specifically
between
telomeric
substrates
;
TRF
2
's
basic
domain
is
particularly
important
for
this
stimulation
.
Although
TRF
1
binds
telomeric
DNA
with
similar
affinity
,
it
has
minimal
effects
on
WRN
-mediated
strand
exchange
of
telomeric
DNA
.
Moreover
,
TRF
2
is
displaced
from
telomeric
DNA
by
WRN
,
independent
of
its
ATPase
and
helicase
activities
.
Together
,
these
results
suggest
that
TRF
2
and
WRN
act
coordinately
during
telomeric
recombination
processes
,
consistent
with
certain
telomeric
abnormalities
associated
with
alteration
of
WRN
function
.
Diseases
Validation
Diseases presenting
"early onset"
symptom
22q11.2 deletion syndrome
alexander disease
alpha-thalassemia
benign recurrent intrahepatic cholestasis
cadasil
canavan disease
coats disease
cohen syndrome
congenital diaphragmatic hernia
dystrophic epidermolysis bullosa
epidermolysis bullosa simplex
erythropoietic protoporphyria
fabry disease
familial mediterranean fever
homocystinuria without methylmalonic aciduria
inclusion body myositis
kindler syndrome
krabbe disease
papillon-lefèvre syndrome
primary hyperoxaluria type 1
pyruvate dehydrogenase deficiency
scrub typhus
sneddon syndrome
triple a syndrome
von hippel-lindau disease
werner syndrome
wolf-hirschhorn syndrome
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