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Multiplexed Analysis of Genes Using Nucleic Acid-Stabilized Silver-Nanocluster Quantum Dots.
[werner syndrome]
Luminescent
nucleic
acid-stabilized
Ag
nanoclusters
(
Ag
NCs
)
are
applied
for
the
optical
detection
of
DNA
and
for
the
multiplexed
analysis
of
genes
.
Two
different
sensing
modules
including
Ag
NCs
as
luminescence
labels
are
described
.
One
sensing
module
involves
the
assembly
of
a
three
-component
sensing
module
composed
of
a
nucleic
acid-stabilized
Ag
NC
and
a
quencher-modified
nucleic
acid
hybridized
with
a
nucleic
acid
scaffold
that
is
complementary
to
the
target
DNA
.
The
luminescence
of
the
Ag
NCs
is
quenched
in
the
sensing
module
nanostructure
.
The
strand
displacement
of
the
scaffold
by
the
target
DNA
separates
the
nucleic
acid-functionalized
Ag
NCs
,
leading
to
the
turned-on
luminescence
of
the
NCs
and
to
the
optical
readout
of
the
sensing
process
.
By
implementing
two
different-sized
Ag
NC-modified
sensing
modules
,
the
parallel
multiplexed
analysis
of
two
genes
(
the
Werner
Syndrome
gene
and
the
HIV
,
human
immunodeficiency
,
gene
)
,
using
615
and
560
nm
luminescent
Ag
NCs
,
is
demonstrated
.
The
second
sensing
module
includes
the
nucleic
acid
functionalized
Ag
NCs
and
the
quencher-modified
nucleic
acid
hybridized
with
a
hairpin
DNA
scaffold
.
The
luminescence
of
the
Ag
NCs
is
quenched
in
the
sensing
module
.
Opening
of
the
hairpin
by
the
target
DNA
triggers
the
luminescence
of
the
Ag
NCs
,
due
to
the
spatial
separation
of
the
Ag
NCs
/
quencher
units
.
The
system
is
applied
for
the
optical
detection
of
the
BRAC
1
gene
.
In
addition
,
by
implementing
two
-sized
Ag
NCs
,
the
multiplexed
analysis
of
two
genes
by
the
hairpin
sensing
module
approach
is
demonstrated
.
Diseases
Validation
Diseases presenting
"immunodeficiency"
symptom
adrenal incidentaloma
allergic bronchopulmonary aspergillosis
cushing syndrome
dracunculiasis
hirschsprung disease
hodgkin lymphoma, classical
homocystinuria without methylmalonic aciduria
kabuki syndrome
legionellosis
malignant atrophic papulosis
oculocutaneous albinism
omenn syndrome
papillon-lefèvre syndrome
primary effusion lymphoma
primary hyperoxaluria type 1
pyomyositis
severe combined immunodeficiency
sneddon syndrome
werner syndrome
wiskott-aldrich syndrome
wolf-hirschhorn syndrome
x-linked adrenoleukodystrophy
This symptom has already been validated
