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Multiplexed Analysis of Genes Using Nucleic Acid-Stabilized Silver-Nanocluster Quantum Dots.
[werner syndrome]
Luminescent
nucleic
acid-stabilized
Ag
nanoclusters
(
Ag
NCs
)
are
applied
for
the
optical
detection
of
DNA
and
for
the
multiplexed
analysis
of
genes
.
Two
different
sensing
modules
including
Ag
NCs
as
luminescence
labels
are
described
.
One
sensing
module
involves
the
assembly
of
a
three
-component
sensing
module
composed
of
a
nucleic
acid-stabilized
Ag
NC
and
a
quencher-modified
nucleic
acid
hybridized
with
a
nucleic
acid
scaffold
that
is
complementary
to
the
target
DNA
.
The
luminescence
of
the
Ag
NCs
is
quenched
in
the
sensing
module
nanostructure
.
The
strand
displacement
of
the
scaffold
by
the
target
DNA
separates
the
nucleic
acid-functionalized
Ag
NCs
,
leading
to
the
turned-on
luminescence
of
the
NCs
and
to
the
optical
readout
of
the
sensing
process
.
By
implementing
two
different-sized
Ag
NC-modified
sensing
modules
,
the
parallel
multiplexed
analysis
of
two
genes
(
the
Werner
Syndrome
gene
and
the
HIV
,
human
immunodeficiency
,
gene
)
,
using
615
and
560
nm
luminescent
Ag
NCs
,
is
demonstrated
.
The
second
sensing
module
includes
the
nucleic
acid
functionalized
Ag
NCs
and
the
quencher-modified
nucleic
acid
hybridized
with
a
hairpin
DNA
scaffold
.
The
luminescence
of
the
Ag
NCs
is
quenched
in
the
sensing
module
.
Opening
of
the
hairpin
by
the
target
DNA
triggers
the
luminescence
of
the
Ag
NCs
,
due
to
the
spatial
separation
of
the
Ag
NCs
/
quencher
units
.
The
system
is
applied
for
the
optical
detection
of
the
BRAC
1
gene
.
In
addition
,
by
implementing
two
-sized
Ag
NCs
,
the
multiplexed
analysis
of
two
genes
by
the
hairpin
sensing
module
approach
is
demonstrated
.
