Expression profile of Caenorhabditis elegans mutant for the Werner syndrome gene ortholog reveals the impact of vitamin C on development to increase life span.

[werner syndrome]

Werner Syndrome (WS ) is a rare disorder characterized by the premature onset of a number of age-related diseases . The gene responsible for WS encodes a DNA helicase /exonuclease protein believed to affect different aspects of transcription , replication , and DNA repair . Caenorhabditis elegans (C . elegans ) with a nonfunctional wrn-1 DNA helicase ortholog also exhibits a shorter life span , which can be rescued by vitamin C . In this study , we analyzed the impact of a mutation in the wrn-1 gene and the dietary supplementation of vitamin C on the global mRNA expression of the whole C . elegans by the RNA-seq technology .Vitamin C increased the mean life span of the wrn-1 (gk 99 ) mutant and the N 2 wild type strains at 25 Â°C . However , the alteration of gene expression by vitamin C is different between wrn-1 (gk 99 ) and wild type strains . We observed alteration in the expression of 1522 genes in wrn-1 (gk 99 ) worms compared to wild type animals . Such genes significantly affected the metabolism of lipid , cellular ketone , organic acid , and carboxylic acids . Vitamin C , in return , altered the expression of genes in wrn-1 (gk 99 ) worms involved in locomotion and anatomical structure development . Proteolysis was the only biological process significantly affected by vitamin C in wild type worms .Expression profiling of wrn-1 (gk 99 ) worms revealed a very different response to the addition of vitamin C compared to wild type worms . Finally , vitamin C extended the life span of wrn-1 (gk 99 ) animals by altering biological processes involved mainly in locomotion and anatomical structure development .

Diseases
Validation

Diseases presenting "mutation in the wrn-1 gene" symptom

werner syndrome

You can validate or delete this automatically detected symptom