Overexpression and amplification of Murine double minute 2 as a diagnostic tool in large lipomatous tumor and its correlation with Ki67 proliferation index: An institutional experience.

[well-differentiated liposarcoma]

Well-differentiated liposarcoma (WDLPS ) is the most common type of liposarcoma and sometimes can be difficult to distinguish from large lipoma due to the similar morphology .This study proposed to evaluate the expression and amplification of Murine double minute 2 (MDM 2 ) gene and determine its correlation with Ki 67 proliferation index .This study used cross-sectional design .This study enrolled 37 cases of lipomatous tumors with >5 cm in size . Eighteen cases of WDLPS and 19 cases of lipoma were stained for MDM 2 and Ki 67 immunohistochemistry , followed by MDM 2 in situ hybridization in 12 selected cases .M DM 2 overexpression and amplification status for both groups were compared using Chi -square test , with the alternative of Fisher 's exact test . Correlation test between MDM 2 overexpression and clinical characteristics with the Ki 67 proliferation index were performed using Pearson 's test with the alternative of Spearman 's rho test .M DM 2 overexpression was detected in all WDLPS cases and in 3 (16 %) of lipoma cases with significance difference (P = 0 .000 ), whereas MDM 2 amplification was found in all WDLPS and in 1 of lipoma cases (P = 0 .200 ). There was a strong correlation between MDM 2 overexpression and higher Ki 67 proliferation index (r = 0 .645 , P = 0 .000 ).Evaluation of MDM 2 overexpression can be used as a useful adjunct to differentiate WDLPS from large lipoma and seems to be related with Ki 67 proliferation index .

Diseases
Validation

Diseases presenting "common type" symptom

alexander disease

cutaneous mastocytosis

dedifferentiated liposarcoma

dentin dysplasia

dentinogenesis imperfecta

erythropoietic protoporphyria

esophageal carcinoma

hereditary cerebral hemorrhage with amyloidosis

liposarcoma

oculocutaneous albinism

oligodontia

pendred syndrome

pleomorphic liposarcoma

pyomyositis

well-differentiated liposarcoma

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