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CD200 flow cytometric assessment and semiquantitative immunohistochemical staining distinguishes hairy cell leukemia from hairy cell leukemia-variant and other B-cell lymphoproliferative disorders.
[waldenström macroglobulinemia]
To
evaluate
CD
200
expression
in
B-
cell
proliferative
disorders
.
We
analyzed
180
recent
specimens
of
B-
cell
neoplasms
for
CD
200
expression
by
flow
cytometric
immunophenotypic
analysis
,
which
is
better
able
to
assess
relative
intensity
of
staining
than
immunohistochemical
staining
.
We
found
that
hairy
cell
leukemia
exhibits
a
high
level
of
staining
for
CD
200
in
comparison
to
other
B-
cell
lymphoproliferative
disorders
,
including
hairy
cell
leukemia
-variant
(
HCL
-V
)
,
marginal
zone
lymphoma
,
and
lymphoplasmacytic
lymphoma
.
We
confirmed
this
observation
by
semiquantitative
immunohistochemical
staining
.
Assessment
of
the
CD
200
expression
level
is
helpful
to
distinguish
HCL
from
HCL
-V
and
other
B-
cell
lymphoproliferative
disorders
and
in
the
differential
diagnosis
of
B-
cell
neoplasms
in
general
.
Diseases
Validation
Diseases presenting
"which is better able to assess relative intensity of staining than immunohistochemical staining"
symptom
waldenström macroglobulinemia
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